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August/September 2010


method. This includes the retention of solutes, the selectivity of the phase system, the resolution of critical peak pairs as well as the polarity of the mobile phase. Temperature programming can be used to replace solvent programming when the composition of the mobile phase cannot be changed. The requirements for high- temperature HPLC are a heating system which should be modular and allows for precise and rapid heating of the mobile and stationary phases. What needs to be considered is the stability of the stationary phase. Besides metal oxide phases which are commercially available for nearly 20 years, the new generation of hybrid materials based on silica has been proven to be extremely stable at temperatures above 100 °C.


References [1] S. Giegold, T. Teutenberg, J. Tuerk, T. Kiffmeyer, B. Wenclawiak, J. Sep. Sci. 31 (2008) 3497.


[2] C.V. McNeff, B. Yan, D.R. Stoll, R.A. Henry, J. Sep. Sci. 30 (2007) 1672.


[3] J.P. Godin, G. Hopfgartner, L. Fay, Anal. Chem. 80 (2008) 7144.


[4] Y. Yang, T. Kondo, T.J. Kennedy, J. Chromatogr. Sci. 43 (2005) 518.


[5] A.R. de Boer, J.M. Alcaide-Hidalgo, J.G. Krabbe, J. Kolkman, C.N. van Emde Boas,W.M. Niessen, H. Lingeman, H. Irth, Anal. Chem. 77 (2005) 7894.


[6] D. Louden, A. Handley, S. Taylor, I. Sinclair, E. Lenz, I.D.Wilson, Analyst 126 (2001) 1625.


[7] T. Teutenberg, S.Wiese, P.Wagner, J. Gmehling, J. Chromatogr. A 1216 (2009) 8480.


[8] T.S. Kephart, P.K. Dasgupta, Talanta 56 (2002) 977. [9] http://www.zirchrom.com/metalox.asp, July 2010. [10] http://www.selerity.com/main/main_products_hplc_ 9000.html, July 2010.


[11] http://www.sim-gmbh.de/index.php?option=com_content &task=view& id=64&Itemid=502&lang=en, July 2010.


[12] T. Teutenberg, J. Tuerk, M. Hozhauser, S. Giegold, J. Sep. Sci. 30 (2007) 1101.


[13] T. Teutenberg, K. Hollebekkers, S.Wiese, A. Boergers, J. Sep. Sci. 32 (2009) 1262.


[14] T. Teutenberg, Anal. Chim. Acta 643 (2009) 1. [15] T. Teutenberg, S.Wiese, P.Wagner, J. Gmehling, J. Chromatogr. A 1216 (2009) 8470.


[16] C.R.Wilke, P. Chang, AIChE J. 1 (1955) 264. [17] M. Krummen, A.W. Hilkert, D. Juchelka, A. Duhr, H.J. Schluter, R. Pesch, Rapid Commun. Mass Spectrom. 18 (2004) 2260.


[18] M. Roloff, H. Erfurt, G. Kindel, C.-O. Schmidt, G. Krammer, Process for the separation and sensory evaluation of flavours,WO2006/111476 A1,World Intellectual Property Organization, Symrise GmbH & Co. KG, Holzminden, Germany, 2006. (Patent)


[19] T. Teutenberg, H.J. Goetze, J. Tuerk, J. Ploeger, T.K. Kiffmeyer, K.G. Schmidt,W.G. Kohorst, T. Rohe, H.D. Jansen, H.Weber, J. Chromatogr. A 1114 (2006) 89.


[20] T. Teutenberg, P.Wagner, J. Gmehling, J. Chromatogr. A 1216 (2009) 6471.


NewMethod Development Service using HPLC Columns


A unique newmethod development service using HPLC columns at the cutting edge of current technology is being offered by Crawford Scientific to customers within the UK.


The technical knowledge base built up by the company over its 25 years existence will be used to improve customers existing separations by whatever criteria they are seeking to obtain improvements. This could be resolution, alternate selectivity, speed of analysis or reduced solvent consumption amongst the possibilities.


Tony Taylor, Crawford’s Technical Director comments “We have found by talking to customers that new technologies such as sub-2µmparticles, superficially porous silica and HILIC phases can cause technology or knowledge barriers within organisations to seeing what actual improvements and benefits can be realised by implementing these columns.”


Tony continued “We are therefore issuing a challenge to customers to submit their existing HPLCmethod and Crawfords will visits the site with a translatedmethod followed by a live demo using a 25%discount column fromour current list. In this way there isminimumdisruption or cost to the customer and everything to gain.” Columns on the 25%discount list include SILECObelisc™and Primesep®mixedmode (HILIC) phases, Agilent Poroshell 120, superficially porous silica column range and the Agilent Zorbax sub-2µmRRHDrange.


Further details on the offermay be obtained directly from


Crawford Scientific, http://www.crawfordscientific.com/method-challenge.html


New Liquid Chromatography Series Offers More Productivity, Confidence, and Affordability


Agilent Technologies announce the 1200 Infinity Series, setting new levels of performance in analytical HPLC and UHPLC. The 1200 Infinity Series includes the new Agilent 1220 Infinity LC, the new Agilent 1260 Infinity LC and the enhanced Agilent 1290 Infinity LC. The Agilent 1200 Infinity Series marks the evolution of the successful 1200 Series LC family and 1120 Compact LCs using latest 1200 Series Rapid Resolution LC and 1290 Infinity LC technologies.


The 1200 Infinity Series portfolio offers a seamless integration of column technology, instrumentation and software to ensure utmost productivity. The 1220 and 1260 Infinity LCs offer enhanced HPLC and RRLC performance at an HPLC price. All 1220 and 1260 Infinity systems are standardized on 600 bar systempressure, 80 Hz data-acquisition speed and 2x or 10x higher UV sensitivity. The 1220 and 1260 Infinity LCs are 100 percent compatible with HPLCmethods, ensuring risk-free replacement or upgrades of existing 1100 or 1200 Series instruments. Further, the new 1260 InfinityQuaternary Bio-inert LC systemoffers the highest performance in bio-analysis and bio-purification.


“We ended the UHPLC debate with last year’s introduction of the Agilent 1290 Infinity LC," said Patrick Kaltenbach, Agilent’s vice president and general manager, Liquid Phase Separations Business. "Now we are raising the HPLC standard with a new portfolio that offers a unique continuumof future-proof HPLC, RRLC and UHPLC solutions tomatch any application or budget.”


The Agilent 1200 Infinity Series portfolio allows customers to tailor the exact systems they need, from the simplest manual isocratic LC through the world's highest-performance, fastest, most sensitive UHPLC systems. The power range of 1200 Infinity Series LC systems is fully compatible with the latest column technology, such as 600 bar Poroshell and Zorbax RRHT or 1200 bar Zorbax RRHD columns.


For more information visit www.agilent.com


To view past issues or the latest news online please visit www.chromatographytoday.com If you would like to be included please email your details or call us on: +44 (0)1727 855574 Produced in association with the Chromatography Society


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