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August/September 2010
the GC-MS/MS ion source, exactly the opposite occurs in an LC-MS/MS API ion source. Until this sampling rate is improved, it appears that GC-MS/MS will be more efficient and this has a direct impact on sensitivity.
Conclusion
Choosing whether to implement LC-MS/MS or GC-MS/MS for bioanalysis depends on the specific application requirements. LC-MS/MS is the preferred technique for simple bioanalysis of an extended range of compound classes and molecular weights from small to large non- volatile molecules. However, the method does not offer the accuracy, precision, specificity and
sensitivity required for specific applications including the analysis of steroids (estrogens and androgens). A highly sensitive GC-MS/MS bioanalytical assay for free testosterone in UF plasma from post-menopausal women has been successfully implemented to help elucidate the pharmacokinetic parameters of testosterone-related therapy and the monitoring of endogenous testosterone as a pharmacodynamic biomarker.
References 1. Gohlke, R.S. and McLafferty, F. W. J. Amer Soc. Mass Spectrom. (1993), 4, 367
2. Yamashita, M., and Fenn, J.B. J. Phys. Chem. (1984) 88, 4671.
3. Herman J. L. Rapid
Commun.Mass Spectrom. (2002) 16, 421. 4. Wang, S., Paris, F., Sultan, C. S., Song, R., Demers, L.N., Sudaram, B., Settlege, J., Ohorodnik, S., and Santen, R. JCEM (2005) 3, 1407.
5. Santen, R., Demers, L., Ohorodnik, S., Settlage, J., Langecker, P., Blanchett, D., Goss, P. E., and Wang S. Steroids (2007) 72, 656.
6. Vesper, H.W., Bhasin, S., Wang, C., Tai. S., Dodge, L.A., Singh, R. J., Nelson, J., Ohorodnik, S., Clarke, N. J., Salameh, W. A., Parker, R. C. Jr., Razdan, R., Monsell, E. A. and Myers, G. L. Steroids (2009) 74, 498.
7. Singh. R. J. AACC (2008) 34, 12. 8. Bonfiglio, R., King, R.C., Olah, T. V., Merkle, K. Rapid Commun. Mass Spectrom. (1999) 13, 1175.
We would like to acknowledge Robert White, Randy Weintraub, Bhaskar Sundaram, James Settlage, and Susan Ohorodnik.
Impressive Speaker Line Up Focuses on Efficiency Improvements
and interest for owners or managers of chromatography laboratories and offers plenty of opportunity to interact with the speakers and network with other delegates. Throughout the two day conference the focus will be on lowering overheads whilst improving data quality.
The full line-up of speakers has now been announced for the 2010 Anatune conference, entitled ‘Automation in the Chromatography Laboratory’. The aim of the conference, which will be held at Down Hall Country House Hotel in Hatfield Heath, UK, on 5-6 October 2010, is to help delegates find innovative ways of improving efficiency in their laboratories. The conference promises to be packed full of inspiration
Speakers for this year’s conference come from the UK, Europe, the Far East and the US; all have a wide and impressive background in analytical technology: Dr Jaroslav Slobodnik, Director of Environmental Institute in Slovakia; Dr Jim Jones, Managing Director of Jones Environmental, Chester, UK; Nobuo Ochiai, Technical Director, GERSTEL Japan; Mr Ray Perkins, Managing Director, Anatune Limited, Cambridge, UK; Mr Bob Green, Technical Director, Anatune Limited, Cambridge, UK; Mr Pierre Alves, Export Technical Sales Manager, OI Analytical, Texas, USA; Mr Angus Macmaster, Analytical Team Leader, Givaudan UK Ltd, Kent, UK; Mr Don Harris, President, ASAP Analytical, Kentucky, USA; and Mr Kevin MacNamara, Head of Technical Development, Irish Distillers Co. Cork, Ireland. Down Hall Country House Hotel, the venue for the conference, is set in 110 acres of beautiful parkland and is easily accessible, being just 15 minutes from the M11 and Stansted airport.
For more information, visit
www.anatune.co.uk
New Standalone Ultrahigh-Pressure Pump for Proteomics Workflows
Using Liquid Chromatography Dionex announce the expansion of its family of industry-leading Rapid Separation LC (RSLC) systems with the new UltiMate® 3000 NCP-3200RS nano/capillary LC pump module. This new standalone ultrahigh-pressure pump features an extensive flow-pressure footprint, delivering continuous, direct flow rates from 20 nL/min to 50 L/min at backpressures of up to 800 bar (11,600 psi) for nano, capillary, and micro LC analyses.
The pump can be used to configure an entry-level RSLCnano system for reversed-phase separation of peptides in LC-MS proteomics workflows. Alternatively, the pump can be used as an add-on module to our industry-leading RSLCnano system for advanced applications, such as automated off-line 2D-LC or tandem nano LC. Tandem nano LC can be an attractive technique for customers who want to improve
their mass spectrometry use and increase sample throughput. Dedicated application kits have been introduced for the RSLCnano system, such as automated off-line RP × RP of peptides and tandem nano LC, to facilitate instrument installation and get effective data from the first experiments.
In addition, Dionex has also released new biocompatible switching valves, featuring a maximum pressure specification of 345 bar (5,000 psi), for sample injection and column switching. These values are intended for use with the RSLCnano system, to provide a biocompatible sample flow path that can be important to customers dealing with the analysis of phosphopeptides.
For more information, visit
www.dionex.com.
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