16
August/September
Since temperature is an instrumental setting, it is one of the easiest and most straightforward parameters to tune chromatographic selectivity. Analyte stability is a major concern when using high temperature in LC. However, many compounds considered thermally labile do not degrade when being analyzed at higher temperatures. If degradation does occur, the amount of breakdown depends not only on the time spent at the higher temperatures, but above all on the nature and quality of the packing material (e.g. presence of trace metals) [5,6]
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References [1] G. Vanhoenacker, A. Dos Santos Pereira, T. Kotsuka, D. Cabooter, G. Desmet, P. Sandra, J. Chromatogr. A 1217 (2010) 3217.
[2] L.R. Snyder, J. Chromatogr. B 689 (1997) 105.
[3] D.V. McCalley, J. Chromatogr. A 902 (2000) 311.
[4] J.W. Dolan, J. Chromatogr. A 965 (2002) 195.
Figure 3. Influence of temperature on the analysis of a mixture Metoclopramide and impurities on a Shodex ET-RP1 column (150 mm L x 4.6 mm ID, 4 µm particles). Flow rate: 0.65 mL/min, gradient: ammonium acetate pH 8.8/ACN, 95/5 hold for 3 min, gradient 95/5 to 5/95 from 3 to 18 min, detection: DAD, 275 nm. Temperature is controlled by Polaratherm 9000 Series column oven equipped with active mobile phase preheating and effluent cooling (SandraSelerity Technologies, Kortrijk, Belgium).
[5] G. Vanhoenacker, P. Sandra, J. Sep. Sci. 29 (2006) 1822.
[6] J. Thompson, P. Carr, Anal. Chem. 74 (2002) 1017.
New Column Oven for Liquid Chromatography
Thermo Fisher Scientific announce the availability of the new Thermo Scientific Column Oven range. Recognizing the importance of temperature as a parameter in liquid chromatography, the Column Oven delivers efficient temperature control coupled with the highest levels of reproducibility and stability.
The effect of temperature on LC separations is often crucial where closely eluting substances are being separated and therefore, where accurate data is required, precise temperatures are vital. With exact temperature control to +/-0.1°C, the Thermo Scientific Column Oven gives scientists the confidence that their oven provides the accuracy that they need, first time; every time. The temperature control unit of Column Oven is designed for simple, fast manual control of the column temperature or alternatively, optional drivers can be used to control the Column Oven through the instrument software to further assist users.
The Column Oven comes in two specifications enabling the user to choose the most appropriate model for their specific application; the Column Oven 200 is capable of accommodating up to 4 analytical HPLC columns with lengths up to 150mm, while the Column Oven 300, is able to contain as many as 4 analytical columns with lengths to 250mm. Either configuration can be mounted on a CTC autosampler or many Thermo Scientific mass spectrometer ion sources.
For more information, visit
www.thermofisher.com
Dani SpA and Jeol Enter
Distribution Agreement Dani SpA, a producer and developer of high level GC systems and attachments for the GC market and Jeol (Europe) BV have signed a distribution agreement for the Benelux countries. As the companies have complimentary systems and do not compete on any level, both Dr Umberto Saini from Dani and Dr Bob Hertsens from Jeol are looking forward to expanding market share with this strategic agreement, a report said. The Master-TOF high throughput GC-TOF developed by Dani can be combined with the JMS-T100GCv from Jeol for example and Q-MS was also said to be available. The companies are expecting that the combination of GC-TOF systems will open possibilities in applications such as fragrances, environmental, petrochemical and food research, as well as quality control and other areas. For more information visit
www.danispa.it
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