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Highlights from


Techniques and Materials Applications T e Infl uence of Beam Broadening on the Spatial Resolution of Annular Dark Field Scanning Transmission Electron Microscopy by N de Jonge, A Verch, and H Demers, Microsc Microanal 24(1) (2018) 8–16.


T e spatial resolution of aberration-corrected annular dark fi eld (ADF) scanning transmission electron microscopy (STEM) was studied as function of the vertical position z within a sample. T e samples consisted of gold nanoparticles (AuNPs) positioned in diff erent horizontal layers within aluminum matrices of 0.6 and 1.0 µm thickness. T e highest resolution was achieved in the top layer, whereas the resolution was reduced by beam broadening for AuNPs deeper in the sample. To examine the infl uence of the beam broadening, the intensity profi les of line scans over nanoparticles at certain vertical locations were analyzed. T e experimental data were compared with Monte Carlo simula- tions that accurately matched the data. T e spatial resolution was also calculated using three diff erent theoretical models of the beam blurring as function of the vertical position within the sample. One model considered beam blurring to occur as a single scattering event but was found to be inaccurate for larger depths of the AuNPs in the sample. Two models were adapted and evaluated that included estimates for multiple scattering. One of these models described the data with suffi cient accuracy to be able to predict the resolution. Beam broadening depended on z 1.5 in the experimental data, a model including multiple scattering and Monte Carlo simulations.


Resolution of STEM measured on AuNPs at different z in a sample consisting of Al layers. Experimental data were compared with different analytical models of beam broadening and with Monte Carlo simulations.


Techniques and Biological Applications


Early Eff ects of Ionizing Radiation on the Collagen Hierarchical Structure of Bladder and Rectum Visualized by Atomic Force Microscopy by SL Kotova, PS Timashev, GV Belkova, MV Kochueva, KV Babak,VA Timofeeva, EB Kiseleva, OO Vasilieva, AV Maslennikova and AB Solovieva, Microsc Microanal 24(1) (2018) 38–48.


Radiation therapy, used in the treatment of pelvic area malignancies, is associated with inevitable damage to the surrounding healthy tissues. We have applied atomic force microscopy (AFM) to track the early damaging eff ects of ionizing radiation on the collagen structures in the rat bladder and rectum. T e fi rst signs of the low-dose radiation (2 Gy) eff ect were detected by AFM as early as 1 week post irradiation. T e observed changes were consistent with initial radiation destruction of the protein matrix. T e alterations in the collagen fi bers’ packing 1 month post irradiation were indicative of the onset of fi brotic processes. T e destructive eff ect of higher radiation doses was probed 1 day post treatment. T e severity of the radiation damage was proportional to the dose, from relatively minor changes in the collagen packing at 8 Gy, to increasing collagen matrix destruction at higher doses, and complete collagen network restructuring toward fi brotic-type architecture at 22 Gy. T e AFM study appeared superior to the light optical microscopy-based studies in its sensitivity to early radiation damage of tissues.


Oriented, tightly packed collagen fi brils forming thick fi bers (signs of fi brosis) in the bladder extracellular matrix 1 month after irradiation at the dose of 2 Gy. Phase image, 6 × 6 µm.


54 doi: 10.1017/S1551929518000445 www.microscopy-today.com • 2018 May


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