NetNotes


very nice, the long working distance presents a problem for long duration time-lapse imaging with multiple positions. Water works fine, but it eventually evaporates (in hours). ZEISS Immersol W does not evaporate but cannot hold the column when the objective is over a mm from the coverslip (it drips/slides off). Leica has an automated water dispenser, but it is not available for my current demo. I was wondering if anyone had any ideas for other immersion options that have a surface tension and RI similar to water, but don’t evaporate as easily. Pablo Ariel pablo_ariel@med.unc.edu


We use Viscotears (artificial tear-fluid replacement) as the


immersion fluid with exactly that lens (and its 10x and 20x relatives) for in vivo imaging of organoids transplanted to the anterior chamber of the eye with great results. Tis should be available from a pharmacy. Tilo Moede tilo.moede@ki.se


Many years ago, we used Suave hair gel. It worked for multiple


positions fairly close together. Gary Laevsky glaevsky.lists@gmail.com A few years ago, people on this listserv suggested jelly for


sonograms and Genteal eye gel. Here is info on the latter http:// microscopynotes.com/710/genteal/index.html Michael Cammer michael.cammer@med.nyu.edu


We have used Genteal Severe Gel (Hypromellose 0.3%) with our


long working distance lens on an inverted system. It lasts overnight but will not last 48 hours without replenishment. For longer experiments, we re-apply during a break in imaging, roughly every 12 hours or so. We also made a little wall out of a glove finger to hold the gel in place and I think this reduces evaporation as well (but it limits ability to do large


Z-stacks as the glove material folds under pressure). Teresa Swayne tcs6@cumc.columbia.edu


water? You can get separate oils for matching water at room temperature (∼27o


Why not use the Cargille oils that match the refractive index of C) or incubation temperature (37o


C). I use these for long-term


imaging without any evaporation issues. Timothy Feinstein tnf8@pitt.edu ZEISS sells oil with a refractive index of 1.33. Tis will go with


any water immersion lenses. We are using this for all of our microscopy systems for extended time-lapse imaging. Ammasi Periasamy ap3t@virginia.edu


Long working distances require that the fluid maintain its structure,


such as with high viscosity or strong surface tension. Te oils run down the side of the lens. Michael Cammer michael.cammer@med.nyu.edu


Tanks all for the many suggestions. I will try the eyedrop


approach and let everyone know if we come up with some sort of containment that can reduce evaporation further. Pablo Ariel pablo_ariel@med.unc.edu


Uniform Focus Across a Field of View Confocal Listserver We have a question about tilt of a specimen or detectors leading


to shiſt of focus across a field of view. We have used many confocal microscopes over the years and have not had problems with the image being in focus corner-to-corner across the field of view when using


Micrograph Awards


To submit your micrographs for the 2022 competition go to the following website:


https://www.microscopy.org/awards/ micrograph_competition.cfm


July cover showing the 25 finalists of the 2021 competition


70


Deadline for the next competition: February 22, 2022


www.microscopy-today.com • 2021 September


Volume 29 Number 4


2021 July


www.microscopy-today.com


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