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Automated Holotomographic Microscopy


Figure 3: Multi-well experiment with the CX-A. (a) Multi-well, time-lapse acquisition allows for drug screening (b) without sacrificing image quality, which gives simultaneous access to (c) a wealth of subcellular details.


are a gold standard for nuclear detection and are broadly used in endpoint


investigations, especially in high-throughput


screens, for the detection of millions of single-cell objects [10]. Te reason is simple: with these easy-to-use and bright dyes, a simple intensity thresholding allows differentiation of true


2021 September • www.microscopy-today.com


signal from background, leading to quick and reliable identifi- cation of unique nuclei as distinct objects [9]. However, the use of DAPI requires sample fixation and


is incompatible with live cell imaging [11], and using other nuclear dyes that are more tolerated by living cells is also


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