48 BIOTECHNOLOGY
Fig. 1. The third-generation PacBio RS incorporates novel, single molecule sequencing techniques and advanced analytics to reveal true biology in real time.
Te science of sequencing is constantly evolving, as more clinical applications are sought and competition with other technologies is resolved. Just one of many trends is that microarrays remain more cost-effective for genome-wide association studies, but given the trends in the science and sequencing costs, that could change too, creating a further boost for DNA sequencers.
New DNA sequencers offer biology in real-time
The market for DNA sequencers is growing, both in the face of demand and in line with the emergence of new, faster technologies. Here Sean Ottewell looks at the latest releases.
Les séquenceurs d’ADN sont un marché grandissant aussi bien face à la demande que sous l’aspect de l’apparition de technologies nouvelles et plus rapides. Sean Ottewell examine les dernières sorties.
Der Markt für DNA-Sequenzer wächst angesichts der Nachfrage und der Entwicklung neuer, schnellerer Technologien. Hier nimmt Sean Ottewell die aktuellen Neuerscheinungen in Augenschein.
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he market for DNA sequencers increased in the last year with the
introduction of new systems and a growing demand for sequencing, according to Kalorama Information.
Te healthcare market research publisher said that the introductions of the Ion Torrent PGM, PacBio RS and MiSeq grew the sequencer market nearly 5 per cent to US$560m (€429m) in 2011. More new technologies are expected from
Ion Torrent as well as from Oxford Nanopore that may further change the market and introduce price changes, according to Kalorama’s report, ‘DNA sequencing equipment and services markets, 3rd edition’.
“Tis was getting to be a stable market, but that changed in the last two years,” said Justin Saeks, Kalorama analyst and author of the report. “Now it’s more volatile and complicated; we think customers will acquire new systems, but pricing and feature preferences will get interesting.”
According to the report, DNA sequencing has seen very high growth in recent years as a result of labs deciding to start using next-generation sequencing for their research. At the same time, large segments of end-users are still continuing to use CE Sanger systems and may take a while to shift to the new technologies.
Another trend is that large structural variations have shown to be highly important for genetic variation, more so than single nucleotide polymorphisms (SNPs); read lengths then become a key factor in system purchases. As specific applications place greater emphasis on one benefit or another, the market segments can quickly narrow down their usage to certain products.
“Longer-term, questions still remain for broad areas such as disease prevention and diagnostics which could turn into home runs or strikeouts,” Saeks said.
The new technologies According to Life Technologies, researchers can now have it all - affordability and full flexibility to perform sequencing of small whole genomes, sets of genes, or transcripts - all on the Ion PGM system.
Different sequencing applications have demands, so in addition to the highly accurate Ion PGM 200 Sequencing Kit, the company has introduced the Ion PGM Sequencing 300 Kit powered with a new long-read sequencing enzyme. Life Technologies says it is committed to further expanding long read sequencing capabilities and expects to support 400-base sequencing by the end of 2012 using the same long-read sequencing chemistry that is in the 300-base kit.
Te Ion PGM Sequencing 300 Kit is designed for de novo and long-
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