26 chromatography • spectroscopy
chromatography system for flexible, reliable and intuitive purification of proteins. ÄKTA pure, the latest addition to the ÄKTA range of chromatography systems, is an easy-to-use modular system that offers the flexibility to support both routine and challenging purification requirements, as well as being adaptable to changing research needs.
Johan von Heijne, head of research products, GE Healthcare Life Sciences, said: “In an increasingly challenging research environment, where needs and priorities can change rapidly, researchers want to know that the protein purification system they purchase has longevity. By taking a modular approach with ÄKTA pure, we are providing a system that not only simplifies researchers’ work but also gives them a flexible option for expanding and tailoring their purification capabilities as their research progresses. Tere is simply an ÄKTA pure for everyone.”
In today’s research environment, scientists are often expected to undertake all aspects of the protein discovery workflow, from genomics through to purification and characterisation, and the requirement to isolate specific proteins at high purity in short timelines often means that chromatography conditions and methodologies have become more challenging. ÄKTA pure
is specifically designed to enable researchers - from beginners to experts - to easily isolate their target protein, whether under simple or complex conditions.
ÄKTA pure eliminates the need for programming skills to control the chromatography run, with the addition of simple drag-and- drop method templates in the UNICORN 6 control software. Trough the software it is also possible to electronically track the use of, and data obtained from, individual columns, which is particularly useful as chromatography systems are often placed in shared facilities.
Ultra inert GC solutions Agilent Technologies has launched a series of new J&W gas chromatography columns designed for environmental and pharmaceutical applications. Te new additions expand the Agilent J&W family of columns, three of which are ultra inert GC solutions.
“Agilent J&W GC columns offer the lowest bleed levels, the best inertness for acids/bases/mixed-function compounds, and the tightest column-to-column reproducibility,” said Anne Jones, general manager, Agilent Chemistries Division. “Every Agilent J&W Ultra Inert GC column is tested with the most demanding ultra inert test-probe mixture in the industry, and an individual performance summary
T
Innovative GC columns
hermo Fisher Scientific offers a range of novel and innovative gas chromatography (GC) columns to meet the changing and
challenging needs of today’s analyses in markets such as environmental, food safety, petrochemical and forensics. However, the company describes its new TraceGOLD columns as a leap forward in GC column technology, offering guaranteed performance. In particular, users benefit from ultra-low bleed, superior inertness and excellent reproducibility.
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sheet is shipped with each column.”
Agilent J&W DB-UI 8270D Ultra Inert GC columns deliver excellent peak-shape performance for active semi-volatile organic compounds targeted by US Environmental Protection Agency method 8270D, so researchers can perform the most challenging environmental trace-level analyses with the utmost confidence.
Te columns are optimised for fast analysis of volatile compounds, and are ideal for environmental and chemical samples with unknown components. Tey deliver the highest degree of column inertness for improved peak shape and linearity for the environmental industry.
Meanwhile Viscotek is the new high temperature gel permeation chromatograph (HT-GPC) from Malvern Instruments. It is a powerful, fully automated, multi-detector system designed to prepare and analyse polymers, such as polyethylene and polypropylene that demand temperatures as high as 160°C in order to dissolve for successful analysis.
Capable of generating molecular weight, molecular weight distribution and structural information in a single experiment, the HT-GPC produces an information-rich data stream for intelligent polymer engineering (Fig. 1).
The company has also officially opened its
molecular biology centre of excellence in Vilnius, Lithuania. The Vilnius site will showcase world-class capabilities for developing and manufacturing products used in a range of life sciences applications. For example, it provides innovative technologies for PCR-based (polymerase chain reaction) testing, nucleic acid sample preparation protein purification and antibodies for ELISA (enzyme-linked immunosorbent assay) testing. “Our expanded R&D and manufacturing
presence in Vilnius strengthens our ability to serve the growing biotechnology community in Eastern Europe, particularly our research customers who rely on innovations in molecular biology to accelerate
Molecular weight and distribution are defining characteristics of polymers, but structure such as the extent of chain branching is also critical. In developing new products, researchers tune vital properties such as strength, processability, flexibility and permeability, by manipulating the extent of branching as well as the molecular weight and distribution. GPC is relied on to provide much of the necessary analytical information.
Te full Viscotek HT-GPC triple detector configuration includes refractive index (RI), viscometry and low angle light scattering (LALS) detection. Together these detectors provide absolute molecular weight without extrapolation or correction, molecular size, intrinsic viscosity and branching information, from a single experiment. Advanced OmniSEC software drives analysis with full polymer branching calculations capability and an intuitive Branching View function that simplifies the study of long chain branching.
Chiral media Phenomenex, a global leader in the research and manufacture of advanced technologies for the separation sciences, has extended its successful Lux chiral media line to meet the needs of preparative chromatography applications. Lux Cellulose-1 and -2, novel and complementary chiral stationary phases, are now available in 10-micron particle size for batch
developments in diagnosing and treating disease,” said Marc N Casper, president and ceo of Thermo Fisher Scientific. “We now have a solid foundation from which we can continue to increase our depth of capabilities for high-growth life sciences markets in the region, from PCR-based products to our broader biosciences portfolio.” The 156,000 ft2
molecular biology centre of
excellence is currently home to more than 400 research, laboratory and manufacturing personnel, with room for expansion. In addition to its geographically central
location, Lithuania is known for its well-established commitment to innovation, highly skilled life sciences workforce and favourable economic environment.
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