46 February / March 2016
mitigated by the dilution in aqueous prior to loading onto the SPE cartridge.
In summary, regardless of the sample preparation technique chosen for whole blood, it is justly important to ensure the analyte-protein interaction is completely broken and that the analyte is released and solubilised into the liquid part of the sample prior to analysis [2].
References:
Figure 20: Comparison of the effects of various pretreatment options on Tramadol, Chromatograms are overlaid with time shift to provide clarity [1].
put forth in this study [2]. This technique does not subject the sample to protein precipitation which helps mitigate loss of analyte due to co-precipitation. This method is also enticing because it does not put forth
any additives that could possibly interfere with the ion exchange mechanism (Zn2+
) or
reduce hydrophobic retention (MeOH and ACN) [2]. However, it is shown in this work that these phenomena are at least partially
1. Sadjadi, Seyed et al. “Comparison of Different Whole Blood Sample Pretreatment Methods for Targeted Analysis of Basic Drugs”. MSACL 2015. Phenomenex. San Diego. April 1st 2015. Poster reference.
2. Simpson, Nigel J K. Solid-Phase Extraction: Principles, Techniques and Applications. New York: Marcel Dekker, 2000.
3. Chen, X-H., Franke, J-P., Wijsbeek J. and deZeeuw, R.A., “Isolation of Acidic, Neutral
Table 3: Method precision and accuracy data based on replicate quality control samples using ZnSO4 and ACN as pretreatment option [1].
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