53 Chromatography — At the Heart of the Olympics

As the 2016 Olympic games get closer, the subject of doping in sport is again one of the main topics of discussion. The recent findings of the McLaren Investigation Report into state controlled doping in Russia show the industrial scale of cheating that some countries and athletes will go to for the chance to win a medal.

During the Olympics in Rio doping control was to take place in the World Anti-Doping Agency (WADA) accredited Brazilian Doping Control Laboratory. But weeks before the games the labs have lost their accreditation due to a non-conformity with the International Standards for Laboratories that WADA uses to endorse labs. While the exact reason is not known yet, it is to be hoped that the lab can regain its accreditation before the games begin.

Athletes who want to compete clean have to abide by the regulations that WADA puts in place. Every year the ‘prohibited’ list is published on the WADA site and it is an athlete’s responsibility to adhere to the regulations. The list includes: substances prohibited at all times, which includes hormones, masking agents and growth factors; substances prohibited in competition, including stimulants and cannabis; substances banned by a particular sport, for example, alcohol is banned in certain sports that involve shooting or driving.

WADA also has a list of methods that are prohibited which includes blood doping, tampering with a sample or urine substitution and genetic manipulation. With hundreds of chemicals on the list, lab testing is demanding, and with so much riding on the results, it is imperative that the labs and their equipment are up to the task.

Labs have to be accredited by WADA, with the standards required for competence and calibration strictly laid down and enforced, although the McLaren Report suggests this is not always true. Accredited labs can use their own methods and are required to carry out research to further the anti-doping fight and make sport fairer and cleaner.

The majority of the testing is carried out using chromatography, with both gas and liquid chromatographs used, depending on the substance under investigation. With so many different molecules to check for, mass spectrometry is now used as the detector of choice, giving a reliable and efficient identification and quantification of the chemicals in each sample.

GC-MS was the mainstay of anti-doping labs for many years, offering reliable and an easy to use methodology. But as the drugs of choice have got more complex, so have the testers and LC-MS offers the drug testers a better chance of catching the cheats, especially with its ability to identify metabolites of drugs in a sample.

every tool you need

With HALO® Columns, You Are Ready for Any Separation Challenge

From legacy HPLC to advanced UHPLC, HALO® columns with Fused-Core®

particle technology

give you all the versatility you need to achieve your separation goals:

• Three Particle Sizes optimised for high ef�cienc� at �our chosen pressures

• Multiple Stationary Phases designed to provide a wide range of separation selectivities

• Different Pore Sizes engineered for small molecules or for peptide, protein and other large molecule separations


Kromasil EternityXT Designed for harsh conditions

From UHPLC to industrial-scale HPLC

Kromasil EternityXT is the latest extension of the Eternity chromatography stationary phases and columns portfolio, for separations and purifications that can be run under a wider range of pH conditions, with particle sizes between 1.8 and 10 µm.

Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60