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1 Contents Head Office ISSN: 1752-8070


International Labmate Ltd. Oak Court, Sandridge Park, Porters Wood, St Albans, Herts AL3 6PH, UK Tel: +44 (0)1727 855574 Fax: +44 (0)1727 841694 info@chromatographytoday.com www.chromatographytoday.com


Michael Pattison, Chairman International Labmate Ltd.


Trevor Hopkins, Editor Chromatography Today Tel: +44 (0)7921 021378 trevor@intlabmate.com


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Editorial Review Board Professor Tony Edge R&D Manager


Agilent Technologies, United Kingdom


Dr Jonathan Crowther Senior Research Fellow


Ortho Clinical Diagnostics, United States Dr Frank P. DiSanzo


ExxonMobil Research & Engineering Analytical Science Laboratory, United States


Ray T. McClain


Associate Principal Scientist Merck & Co. , Inc., United States


Dr Lucie Nováková Associate Professor


Charles University, Faculty of Pharmacy, Hradec Králové, Czech Republic


Dr Matthew Przybyciel


Vice President and Technical Director ES Industries, United States


Professor Torgny Fornstedt Analytical Chemistry


Department of Engineering and Chemical Sciences Karlstad University, Sweden


Dr Richard Henry Consultant, United States


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Rozing.com, Germany


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Poly LC. United States Production Team


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Published – February/March, May/June, August/September and November/December


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Published by International Labmate Ltd. Printed in the UK by Warners Midlands plc.


56 Separation Science / Spectroscopy Meetings Calender


54 Chromatography Today Help Desk Important Considerations for Repeatable HPLC Assays of Chiral Compounds


48 Sub-2µm High Performance Enantiomeric Separations on Whelk-O1


Chiral Stationary Phase


44 Chiral Amino Acid and Peptide Separations – the Next Generation


36 Resurgence in Fluorinated Chiral Polysaccharide Phases for Supercritical


Fluid Chromatography 32 Trace 56 Fe, 63/65 Cu and 64 Zn Metal Analysis in Whole Blood by SEC-HPLC-ICPMS


30 Practical Effects of Sample Diluent on Peak Shape using sub 2um Columns


in Achiral SFC.


23 Simple Method Development for the Separation of Chiral Synthetic Cannabinoids


using Ultra High Performance Supercritical Fluid Chromatography


16 Injection by Extraction in Preparative SFC; Isolation of Impurities and Natural Products


10 Peak Distortions in Preparative Supercritical Fluid Chromatography – a More Complete


Overview Over Years


2 Foreword by Trevor Hopkins Articles


4 How to Design a Column Screening Kit for Preparative SFC Separations?


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