Carmichael’s Concise Review
Coming Events 2016
Physics and Chemistry of Semiconductor
Surfaces and Interfaces (PCSI-43) January 17–21, 2016 Location: Palm Springs, CA
www.pcsiconference.org
24th Australian Conference on
Microscopy and Microanalysis January 31–February 4, 2016 Melbourne, Australia
www.acmm2016.org
Nanoscience and Nanotechnology
(ICONN) 2016 February 7–11, 2016 Canberra, Australia
www.ausnano.net/iconn2016
60th Annual Meeting, Biophysical Society
February 27–March 2, 2016 Los Angeles, CA
www.biophysics.org/Meetings/AnnualMeeting/ tabid/85/
Default.aspx
PITTCON Conference March 6–10, 2016 Atlanta, GA
http://pittcon.org
2016 MRS Spring Meeting March 28–April 1, 2016 Phoenix, AZ
www.mrs.org/spring2016
Microscopy & Microanalysis 2016 July 24–28, 2016 Columbus, OH
www.microscopy.org
European Microscopy Congress August 28–September 2, 2016 Lyon, France
http://emc2016.fr
2017
Microscopy & Microanalysis 2017 July 23–27, 2017 St. Louis, MO
www.microscopy.org 2018
Microscopy & Microanalysis 2018 August 5–9, 2018 Baltimore, MD
www.microscopy.org
2019
Microscopy & Microanalysis 2019 August 4–8, 2019 Portland, OR
www.microscopy.org 2020
Microscopy & Microanalysis 2020 August 2–6, 2020 Milwaukee, WI
www.microscopy.org
More Meetings and Courses Check the complete calendar near the back of this magazine.
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Figure 1: IsoView whole-animal functional images of Drosophila embryos. The length of the embryos is approximately 500 μ m. The two images on the left are maximum-intensity projections of deconvo- luted image data of a stage 17 embryo expressing the calcium indicator GCaMP6s throughout the nervous system. The two images on the right are four-view two-color images of gastrulating embryos with labeled nuclei (His2Av-mRFP1) and membranes (Spider-GFP) using a combination of two IsoView modes.
doi: 10.1017/S1551929515001182 2016 January
Light-sheet microscopy methods have recently been developed for high- speed imaging but have limitations, such as limited penetration, that limits specimen volume size. Powerful strategies have been proposed for improving resolution in light-sheet microscopy, but each has its limitations, such as increased photo-damage that restricts observation times of dynamic events. More recently Raghav Chhetri, Fernando Amat, Yinan Wan, Burkhard Höckendorf, William Lemon, and Philipp Keller have developed another strategy for resolution enhancement that is based on multiview imaging. Their method provides uniform spatial resolution in all dimensions (isotropy) and is labeled IsoView light- sheet microscopy. IsoView light-sheet microscopy rapidly images large specimens via simultaneous light-sheet illumination and fluorescence detection along four orthogonal directions. Combining these four views by means of high-throughput multiview deconvolution yields images with high resolution in all three dimensions.
The specimen is imaged along different directions, which yields different relative orientations. Whereas two views give good resolution, Chhetri et al. designed a microscope with four orthogonal arms for simultaneous light-sheet illumination and fluorescence detection. This yields a massive number of data points that require correspondingly massive computing power, yet these authors
More Views Give Better Spatial and Temporal Resolution of Whole Organisms
Stephen W. Carmichael Mayo Clinic , Rochester , MN 55905
carmichael.stephen@
mayo.edu
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