Volume 22 Number 1 January 2016
table of contents preview BIOLOGICAL APPLICATIONS
Time-Lapse Evaluation of Interactions Between Biodegradable Mg Particles and Cells Florencia Alvarez, Rosa M. Lozano Puerto, Blanca Pérez-Maceda, Claudia A. Grillo, and Mónica Fernández Lorenzo de Mele
Intracellular Biosynthesis of Fluorescent CdSe Quantum Dots in Bacillus subtilis: A Strategy to Construct Signaling Bacterial Probes for Visually Detecting Interaction Between Bacillus subtilis and Staphylococcus aureus
Zheng-Yu Yan, Xiao-Xia Ai, Yi-Long Su, Xin-Ying Liu, Xiao-Hui Shan, and Sheng-Mei Wu Calcium Deposits in the Crayfi sh, Cherax quadricarinatus: Microstructure versus Elemental Distribution
Gilles Luquet, Yannicke Dauphin, Aline Percot, Murielle Salomé, Andreas Ziegler, Maria S. Fernández, José L. Arias
Microscopy and Microanalysis of Blood in a Snake Head Dey et al.
Changes in Synapsin Levels in the Millipede Gymnostreptus olivaceus Schubart, 1944 Exposed to Diff erent Concentrations of Deltamethrin
Annelise Francisco, Pablo H. Nunes, Roberta F. C. Nocelli, Carmem S. Fontanetti Comparison of Aortic Collagen Fiber Angle Distribution in Mouse Models of Atherosclerosis Using Second-Harmonic Generation (SHG) Microscopy
Shana R. Watson, Piaomu Liu, Edsel A. Peña, Michael A. Sutton, John F. Eberth, and Susan M. Lessner
Innovative High Gas Pressure Microscopy Chamber Designed for Biological Cell Observation Mélanie Ragon, Hue N. T i Minh, Stéphane Guyot, Pauline Loison, Gaëtan Burgaud, Sébastien Dupont, Laurent Beney, Patrick Gervais, and Jean-Marie Perrier-Cornet
MATERIALS APPLICATIONS
Quantitative Energy-Dispersive X-Ray Analysis of Catalyst Nanoparticles Using a Partial Cross Section Approach
Katherine E. MacArthur, T omas J. A. Slater, Sarah J. Haigh, Dogan Ozkaya, Peter D. Nellist, and Sergio Lozano-Perez
Scanning Electron Microscope–Cathodoluminescence Analysis of Rare-Earth Elements in Magnets Susumu Imashuku, Kazuaki Wagatsuma, and Jun Kawai
Application of SEM/EDS for characterization of detrital minerals in karst cave speleothems Nina Zupančič, Miloš Miler, Stanka Šebela, and Simona Jarc
Phase Identifi cation of Dual Phase (DP980) Steels by EBSD and Nanoindentation Techniques Fan Zhang, Annie Ruimi, and David P. Field
Photoluminescence Studies of Both the Neutral and Negatively Charged Nitrogen-Vacancy Center in Diamond
Kaiyue Wang, John W. Steeds, Zhihong Li, and Yuming Tian
Dopant-site Determination in Y- and Sc-Doped (Ba0.5Sr0.5)(Co0.8Fe0.2)O3-δ by Atom Location by Channeling Enhanced Microanalysis and the Role of Dopant Site on Secondary Phase Formation Matthias Meff ert, Heike Störmer, and Dagmar Gerthsen Characterization of Al2O3 in High-Strength Mo Alloy Sheets by HRTEM Yucheng Zhou, Yimin Gao, Shizhong Wei and Yajie Hu
FIB Plan View Preparation and Electron Tomography of Ga-Containing Droplets Induced by Melt-Back Etching in Si
Katharina I. Gries, Katharina Werner, Andreas Beyer, Wolfgang Stolz, and Kerstin Volz
T ree-Phase 3D Reconstruction of a LiCoO2 Cathode via FIB-SEM Tomography Zhao Liu, Yu-chen Karen Chen-Wiegart, Jun Wang, Scott A. Barnett, Katherine T. Faber Microstructure of Concrete with Aggregates from Construction and Demolition Waste Recycling Plants
Miguel Bravo, A. Santos Silva, Jorge de Brito, and L. Evangelista Fractal and Lacunarity Analyses: Quantitative Characterization of Hierarchical Surface Topographies
Edwin J. Y. Ling, Phillip Servio, and Anne-Marie Kietzig
In Situ Ptychography of Heterogeneous Catalysts using Hard X-Rays: High Resolution Imaging at Ambient Pressure and Elevated Temperature
Sina Baier, Christian D. Damsgaard, Maria Scholz, Federico Benzi, Amelie Rochet, Robert Hoppe, Torsten Scherer, Junjie Shi, Arne Wittstock, Bitta Weinhausen, Jakob B. Wagner, Christian G. Schroer, and Jan-Dierk Grunwaldt Microscopy and Cathodoluminescence Spectroscopy Characterization of Quartz Exhibiting Diff erent Alkali–Silica Reaction Potential
Aneta Kuchařová, Jens Götze, Šárka Šachlová, Zdeněk Pertold, and Richard Přikryl TECHNIQUES, SOFTWARE, AND EQUIPMENT DEVELOPMENT
Auto Focus by Bayes-Spectral-Entropy Applied to Optical Microscopy Steff en Podlech
Automatic Detection of Pearlite Spheroidization Grade of Steel using Optical Metallography Naichao Chen, Yingchao Chen, Jun Ai, Jianxin Ren, Rui Zhu, Xingchi Ma, Jun Han, Qingqian Ma
Monte Carlo Simulations of Electron Energy-Loss Spectra with the Addition of Fine Structure from Density Functional T eory Calculations
Mohammed Attarian Shandiz, Maxime J.-F. Guinel, Majid Ahmadi, and Raynald Gauvin
T eoretical and Experimental X-ray Peak/Background Ratios and Implications for Energy Dispersive Spectrometry in the Next Generation Analytical Electron Microscope Nestor J. Zaluzec
High Dynamic Range Pixel Array Detector for Scanning Transmission Electron Microscopy Mark W. Tate, Prafull Purohit, Darol Chamberlain, Kayla X. Nguyen, Robert M. Hovden, Celesta S. Chang, Pratiti Deb, Emrah Turgut, John T. Heron, Darrell G. Schlom, Daniel C. Ralph, Gregory D. Fuchs, Katherine S. Shanks, Hugh T. Philipp, David A. Muller, Sol M. Gruner
A Simple TEM Method for Fast T ickness Characterization of Suspended Graphene and Graphite Flakes
Sultan Akhtar, Stefano Rubino and Klaus Leifer
Microscopy and Microanalysis website: http:
journals.cambridge.org/MAM Indexed in Chemical Abstracts, Current Contents, BIOSIS, and MEDLINE (PubMed)
2016 January •
www.microscopy-today.com Dear Abbe,
Help! Like many modern microscopists, I am seriously overworked. Between writing grants and contracts, reviewing other peoples’ grants and contracts, staff meetings, progress reports, committee meetings, time-use reports, writing papers, reviewing papers, and other meetings, it seems the only time I have to do actual microscopy is during my commute. What can I do? Harried in Harrisburg
Dear Harried,
Quit your whining! In my day we had microscopes even in our bathrooms so we could always be working. But a solution is coming soon. As you may be aware, auto manufacturers have been working on self-driving cars. Since many preparation instruments and most microscopes are becoming self-driving, Abbe Labs has been diligently working on a hybrid solution for microscopists. T e microscopy instruments are contained in a self-driving car, and there you are! A whole new kind of micro-car! We call it the Microsetta.
Dear Abbe, I’m using old steel re-sharpenable blades to section
decalcifi ed teeth. I realize most people use disposable blades these days. My concern is that people might think I’m a histology hipster. I get funny looks when people see me using the automatic knife sharpener. When people see me using an old AO 820 microtome, they think I’m some kind of historical histology re-enactor. Should I use the modern equipment we have available in our lab, or should I go full hipster historical histologist on them? Hysterical in Rootstown
Dear Hysterical,
Ach, ja, I see the problem. You are nothing but a plausible poser. “Automatic knife sharpener” indeed! True hard-core histologists hand-sharpen their microtome knives and fi nish them on a leather strop. I call that a true Historical Histological Hipster. When I learned to section, we would travel to the forges of Volund (I recovered his wife’s ring, and he owed me a favor) and forge our own microtomy knives. And woe be to the unfortunate student who couldn’t slice a fresh liver biopsy into 1 µm serial sections with the new knife! Look closer at that “modern equipment”! You’ll fi nd it’s just smoke and mirrors. Go to any histology lab, ignore the lab techs, and look for the real histologist behind the curtain. You’ll fi nd an 820, a proper selection of sharpening stones, a well-cared-for leather strop, and a harassed Histowizard snarling at the Landstreicher who dares disturb his slicing. Say “No” if they ask if you want a slice of meat pie.
Herr Abbe can’t wait to hone his wisdom on the strop of sarcasm! Don’t hesitate to send him a line at
jpshield@uga.edu .
doi: 10.1017/S1551929515001091 65
Dear Abbe
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68