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MICROSCOPY 69


NEW TOOL for enhanced CRYO-CORRELATIVE MICROSCOPY L


inkam Scientifi c Instruments has recently launched the CMS196M for enhanced cryo-


correlative microscopy with greatly improved workfl ow. The company has been developing cryo stages for correlative microscopy for many years and continues to be at the forefront of cryo-correlative microscopy with the latest update of its Link software for the CMS196M, providing improved imaging capabilities and the new liquid


nitrogen autofi ll system providing longer run times.


Cryo-correlative microscopy has


become an established technique in recent years. It brings together the strengths of both electron and light (fl uorescence) microscopy. Electron microscopy (EM) provides structural information at very


high resolution. However, it can give only restricted insight into biological and chemical processes due to limitations in staining and sample preparation processes. On the other hand, fl uorescence microscopy is an extremely sensitive technique to detect biological, chemical and genetic processes as well as events inside living cells. Cryo-Clem brings it all together:


combining the individual advantages from both fl uorescence and EM by imaging the same sample location with both techniques


and superimposing the complementary


information. For biological samples to be compatible with the vacuum


conditions found in EM and preserve the structural detail, samples are embedded in vitrifi ed ‘glass-like’ ice and need to be kept below -140°C. Any contact with moisture contained in the air has to be avoided


since ice crystals would form immediately and contaminate the sample. Under cryo conditions, the fl uorescence signals providing structural detail is preserved and photo bleaching is signifi cantly reduced. Link software for the CMS196 provides


complete control and monitoring of the system as well as fully automated tiled image capture. The integrated, encoded, high-resolution motorised XY stage gives the ability to capture an image of the entire EM grid at full resolution. The system produces a single image of the EM grid that can then be used to navigate the sample and save co-ordinates of areas of interest making it easy to image the same area when the sample is transferred to the EM. In addition, Link can now be supplied with an extremely cost eff ective, high sensitivity monochrome camera ideal for low light fl uorescence imaging.


For more information visit www.linkam.co.uk


on the pco.edge family


the most versatile sCMOS camera portfolio for microscopy applications


cutting edge


GByte/s image data bandwidth


1.1


up to 82%


quantum efficiency


pco.de pco-tech.com


www.scientistlive.com


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