SPECTROSCOPY 47
Seeking regulatory approval for your glycosylated biopharmaceutical?
Fig. 3. CrysAlisPro data collection and reduction software
users. T e simple design also means minimal maintenance and no special infrastructure requirements, meaning that the system can run with very little downtime for servicing. T is is important for universities or service laboratories with a high throughput of samples.
HPC dectector T e new HyPix-Bantam detector is a next-generation two-dimensional semiconductor detector designed specifically to meet the needs of the home lab diff ractionist. It is an HPC detector with a large active area of approximately 3,000mm2
,
a small pixel size of 100 µm2 ×100 µm, and a high count rate of greater than 106
cps/pixel.
A big advantage of HPC detectors is that each pixel is independent and the overall dynamic range of a detector is the sum of the dynamic range of each individual pixel. Each pixel of the HyPix-Bantam has two 16-bit counters, and these can be combined to work as a single 31-bit counter, achieving very wide dynamic range. Another benefit of HPC detectors is the extremely low background noise and high sensitivity, meaning that even low photon counts are detected. T e combination of all these
features means that images with both strong and weak reflections present are measured extremely accurately without the need to do a second pass of
reciprocal space to recollect the image at a lower exposure time. T e nerve centre of the XtaLab mini II system is the CrysAlisPro
software, which ties
all the new detector features together through a highly parallelised architecture, resulting in a reliable system for generating 3D structures of crystalline materials. It is a comprehensive but user-friendly, semi-automated software package, ideal for both researcher and student level users. It links directly to Olex2, a freely available software package for structure solution and refi nement.
Applications In a recent application data was collected on the XtaLab mini II system at room temperature on a crystal of cytidine with dimensions 0.167 × 0.171 × 0.231mm. T e exposure time was set to 10 seconds per frame with 648 images collected in just under 1 hour 50 minutes. Excellent data was collected on this light organic sample in less than two hours using the new diff ractometer. T e HyPix- Bantam detector allows data to be collected very quickly due to its high sensitivity and dynamic range.
Dr. Claire Morgan Head of Business Dev.
claire.morgan@
ludger.com
Paul Swepston is with Rigaku.
www.rigaku.com
Dr. Louise Royle
Head of Glycoprofiling
louise.royle@
ludger.com
www.ludger.com Ludger Ltd, Oxfordshire UK
www.scientistlive.com
Ludger’s expertise and QbD approach to drug glycosylation analysis can help you achieve success. We work with over half of the world’s top pharma companies* *GEN, 2016
Glycoprofiling
Services Detailed glycan characterization using UHPLC, MS and LC-MS and method transfer
Technology Reliable tools for glycan sample preparation, including quantitative standards, clean up and labelling kits
Medical Glycomics Collaborative R&D programmes to study glycosylation in disease and for biomarker discovery
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