www.americanlaboratory.com AL Volume 48, Number 2 | March 2016 FEATURED ARTICLES
Using XPS and a Multi-Mode Ion Source to Understand Advanced Layered Materials ......................................... 12
by T. Nunney A monatomic and gas cluster ion source for XPS instruments uses both monatomic argon ion and argon gas cluster ion sputtering to create depth profi les to understand layer interactions in advanced materials.
Improved Liquid Biopsies With Combined Digital PCR and Next-Generation Sequencing ................................. 17
by G. Karlin-Neumann
Digital droplet PCR and NGS will allow physicians to monitor the effi cacy of treatment in close to real time and identify cancer recurrence earlier.
Determination of Sulfur Compounds in Natural Gas and Gaseous Fuels by Gas Chromatography and Pulsed Flame Photometric Detection ................................................ 20
On the Cover Resorcinal and methylene blue crystal. Technique: polarized light; 33×. Image
captured by Dr. John Hart, University of Colorado Boulder, Department of Atmospheric and Oceanic Sciences, Boulder, Colorado, U.S.A. Image of Distinction in the 2015 Nikon Small World Photomicrography Competition. For more information, visit www.
nikonsmallworld.com.
ULTRA PRECISE PIEZO-Z FOCUSING STAGE
by C. Elmore and M. Duff y
Individual volatile sulfur-containing compounds are determined in gaseous fuels by GC and pulsed fl ame photometric detection.
Super-Resolution Microscopy Helps Extend Optical Observation Beyond Its Theoretical Boundaries ............ 24
by R. Ulbrich The next step in super resolution may allow researchers to image faster-moving, ever- smaller structures for longer periods, even deeper within tissue.
Smart Sample Prep in Chromatography: An Automated Liquid–Liquid Extraction Technique for Metabolomics Analysis .............................................28
by E. Varesio, S. Jahn, S. Cudré, G. Hopfgartner, R. Picenoni and G. Boehm
Fully integrated automation using an integrated robotic system can transform even complex chromatography protocols.
Screening Live Cells Using RNA Detection Probes ........... 32 by H. Lahm and D. Weldon
The RNA detection probes described allow live-cell RNA detection in a single incubation step using inert nanoparticle technology to detect native RNA.
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AMERICAN LABORATORY 2
A Novel Hydrogel-Based Approach for Cell Isolation, Purifi cation and Release .............................................. 40
by A. Armstead Magnetic separation and dissociation leave isolated target cells completely label free for scalable downstream culture, analysis and functional studies.
Long Reads and the Return of Reference Genomes ........ 42 by C. Boles and J. Korlach Long-read sequencing has made it possible to achieve reference-grade genome assemblies.
Long-Range Sequencing is Required to Unlock the Full Genome ............................................................... 44
by M. Schnall-Levin
A sequencing platform provides long-range information from sequencing data and partitions large DNA molecules into droplets.
MARCH 2016
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