“


WE’RE BRINGING A NEW PERSPECTIVE TO


cancer metabolism research FIRST WE MADE IT POSSIBLE – NOW WE’VE MADE IT EASY.


XF technology provides the easiest and most comprehensive assessment of cancer cell metabolism, measuring glucose and glutamine metabolism, and fatty acid oxidation of cancer cells in a microplate, in real-time!


”


— David Ferrick, PhD, CSO, Seahorse Bioscience


Glycolytic Function GlucoseOligomycin2-DG


35 40 45


10 20 30


15 25


Glycolysis


0 5


10


020406080 TIME (minutes)


30


Non-glycolytic Acidification 50


OXPHOS Pathway Inhibition


100 120 140 160 180 200


20 40 60 80


0


0 0.008 0.040.2 15 Oligomycin (µM)


OCR ECAR ATP


20 40 60 80 100 120 140 160 180 200


0


100 120 140 160 180 200


20 40 60 80


0


00.8 42050100 200 2-DG concentration (mM)


70


OCR ECAR ATP


Glycolytic Reserve


Glycolytic Capacity


90 100 Glycolytic Pathway Inhibition


20 40 60 80 100 120 140 160 180 200


0


The Seahorse XFe


Extracellular Flux Analyzer


Measurements of cellular glycolysis are essential to understanding cancer, immune response, stem cell differentiation, aging, and cardiovascular and neurodegenerative diseases. The XFe Analyzers and XF Glycolysis Stress Test Kit make it easy to measure the three key parameters of cellular glycolysis in a microplate: glycolysis, glycolytic capacity, and glycolytic reserve, revealing critical information not evident in mitochondrial respiration measurements alone.


See what’s possible. Scan this QR code to view videos and see what the XF Analyzer can achieve. Visit www.seahorsebio.com/ddw for more information!


% of baseline rate


ECAR (mpH/min)


% of control ATP level % of baseline rate


% of control ATP level


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