Genomics
sequencing with only 10ng of input DNA, has now expanded beyond the 2012 releases of fixed panels for cancer and inherited disease and the ability to design custom panels. The most recent Ion AmpliSeq™ technology launches in 2013 expand the DNA offering to community-designed DNA panels, including designs for colon/lung cancer and BRCA1/2, along with the ability to design custom mouse panels. The launch of the Ion AmpliSeq™ technology for targeted RNA sequencing includes panels for cancer and apoptosis, along with the ability to design custom human RNA panels. The Ion Proton™ System is capable of sequencing 1-2 exomes or 1-4 transcriptomes on the current Ion PI™ Chip. The Ion PII™ Chip, due to begin ship- ping in mid 2013 will scale to provide human-scale genome sequencing in a single day for $1,000. Ion Torrent offers a comprehensive range of sample preparation kits and automation and seamless soft- ware integration for the fastest path to biological results (Figure 14).
Oxford Nanopore Technologies® (
www.nano
poretech.com) is the leading developer of nanopore sensing technology for the analysis of DNA, RNA, proteins and other single molecules. Oxford Nanopore’s new generation of real-time sequenc- ing technology uses nanopores to deliver ultra long read length single molecule sequence data, at com- petitive accuracy. Oxford Nanopore intends to commercialise ‘strand sequencing’ for DNA analy- sis on its scalable electronic GridION platform and offer a miniaturised version of the technology, MinION, which will make nanopore sequencing universally accessible. Oxford Nanopore’s GridION system consists of scalable instruments (nodes) used with consumable cartridges that con- tain proprietary array chips for multi-nanopore sensing. Each GridION node and cartridge is ini- tially designed for real-time sequencing by 2,000 individual nanopores at any one time and will deliver tens of Gb of sequence data per 24-hour period. Alternative configurations with more pro- cessing cores (more than 8,000 nanopores) will become available later. Nodes may be clustered in a similar way to computing devices, allowing users to increase the number of nanopore experiments being conducted at any one time if a faster time-to- result is required. For example, a 20-node installa- tion using an 8,000 nanopore configuration would be expected to deliver a complete human genome in 15 minutes. Pricing will be competitive with other leading systems at launch. Oxford Nanopore has also miniaturised these devices to develop the MinION; a disposable DNA sequencing device the
Drug Discovery World Spring 2013
Figure 15: The Oxford Nanopore Technologies GridION™ system
Figure 16: The Oxford Nanopore Technologies MinION™ device
size of a USB memory stick whose low cost, porta- bility and ease of use are designed to make DNA sequencing universally accessible. A single MinION is expected to retail at less than $900. Orders are not yet being taken for either system, however you may register your interest at its web- site. Oxford Nanopore has an intellectual property portfolio of more than 300 issued patents and
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