Volume 17
Number 4 August 2011 Dear Abbe table of contents preview
Stem Cell Special Issue Introduction to Stem Cell Special Section Heide Schatten
Pluripotent Stem Cells and Reprogrammed Cells in Farm Animals Monika Nowak-Imialek, Wilfried Kues, Joseph W. Carnwath, and Heiner Niemann
How Can Female Germline Stem Cells Contribute to the Physiological Neo-Oogenesis in Mammals and Why Menopause Occurs? Antonin Bukovsky
Te Significant Role of Centrosomes in Stem Cell Division and Differentiation Heide Schatten and Qing-Yuan Sun
Te Hair Follicle Bulge: A Niche for Adult Stem Cells Hilda Amalia Pasolli
Neural Stem Cells in Neurospheres, Embryoid Bodies, and Central Nervous System of Human Embryos A. Henry Sathananthan
Hematopoietic Derived Cell Infiltration of the Intestinal Tumor Microenvironment in ApcMin/+ Mice Celestia Davis, Robert Price, Grishma Acharya, Troy Baudino, Tomas Borg, Franklin G. Berger, and Maria Marjorette O. Peña
Multiphoton Flow Cytometry to Assess Intrinsic and Extrinsic Fluorescence in Cellular Aggregates: Applications to Stem Cells David G. Buschke, Jayne M. Squirrell, Hidayath Ansari, Michael A. Smith, Curtis T. Rueden, Justin C. Williams, Gary E. Lyons, Timothy J. Kamp, Kevin W. Eliceiri, and Brenda M. Ogle
Biological Applications Age-Dependent Expression of Collagen Receptors and Deformation of Type I Collagen Substrates by Rat Cardiac Fibroblasts Christopher G. Wilson, John W. Stone, Vennece Fowlkes, Mary O. Morales, Catherine J. Murphy, Sarah C. Baxter, and Edie C. Goldsmith
Quantitative Electron Microscopy of Cellulose Nanofibril Structures from Eucalyptus and Pinus radiata Kraſt Pulp Fibers Gary Chinga-Carrasco, Yingda Yu, and Ola Diserud
Karyotype Analysis of Buckwheat Using Atomic Force Microscopy Suresh Neethirajan, Tamaki Hirose, Junichi Wakayama, Kazumi Tsukamoto, Hiroko Kanahara, and Shigeru Sugiyama
Materials Applications Compositional Analysis with Atomic Column Spatial Resolution by 5th-Order Aberration-Corrected Scanning Transmission Electron Microscopy David Hernández-Maldonado, Miriam Herrera, Pablo Alonso-González, Yolanda González, Luisa González, Jaume Gazquez, María Varela, Stephen J. Pennycook, María de la Paz Guerrero-Lebrero, Joaquín Pizarro, Pedro L. Galindo, and Sergio I. Molina
Carbon Diffusion from Methane into Walls of Carbon Nanotube through Structurally and Compositionally Modified Iron Catalyst Michael J. Behr, K. Andre Mkhoyan, and Eray S. Aydil
Multifrequency Atomic Force Microscopy: Compositional Imaging with Electrostatic Force Measurements Sergei Magonov and John Alexander
Technology and Software Development Light and
Confocal Microscopy Quality Assurance Testing for Modern Optical Imaging Systems Robert F. Stack, Carol J. Bayles, Anne-Marie Girard, Karen Martin, Cynthia Opansky, Katherine Schulz, and Richard W. Cole
Restoration of Uneven Illumination in Light Sheet Microscopy Images Mohammad Shorif Uddin, Hwee Kuan Lee, Stephan Preibisch, and Pavel Tomancak
Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy Sherry G. Clendenon, Pamela A. Young, Michael Ferkowicz, Carrie Phillips, and Kenneth W. Dunn
Measurements Validating the Confocal Scanning Laser Holography Microscope Peter B. Jacquemin and Rodney A. Herring
Helium Ion Microscopy Angular Dependence of the Ion-Induced Secondary Electron Emission for He+ and Ga+ Beams Vincenzo Castaldo, Josephus Withagen,Cornelius Hagen, Pieter Kruit, and Emile van Veldhoven
Resolution Limits of Secondary Electron Dopant Contrast in Helium Ion and Scanning Electron Microscopy Mark Jepson, Xiong Liu, David Bell, David Ferranti, Beverley Inkson, and Cornelia Rodenburg
Is Microanalysis Possible in the Helium Ion Microscope? David C. Joy and Brendan J. Griffin
BOOK REVIEW Unseen Companions: Big Views of Tiny Creatures Elaine C. Humphrey
2011 July •
www.microscopy-today.com
Dear Abbe, I have a user who insists on growing cells in plastic well plates, and I am trying to come up with a good way to punch or cut out individual wells without disturbing the cells too much. Te idea is to put them on a glass slide for the microscope. Is there a tool that works well on 96- and 384-well plates? I tried to get them to use glass so imaging on our microscope would be easier, but they are stubborn about using plastic. Earl from Corning, NY
Dear Earl-enmyer, I’m afraid I have no tool that works well on 96 or any other number of wells for cutting out the wells. Maybe using a precise painting of acetone along the edges would work? My forte is more along the lines of punching them out. It is a little known fact that I became interested in the pugilistic arts and sparred with Max Schmeling. Max and I became good friends at a Bierschenke aſter a row with some unruly patrons. Aſter sparring mentally with my colleagues, a physical outlet was welcomed. Of course, everyone knows the long-standing relationship of microscopists and boxing, and this has been the basis of many sample techniques. For example, the “Gentleman Jim” of cryo-EM fame is named in honor of American boxer James Corbett. Just take out your fingerling boxing gloves and punch those little devils right out. A few cells might be offended by your violent method, but they’re going to be unhappy under the scope anyway.
Dear Abbe, Would you like to become a millionaire? If so, you should invest the massive royalties from your syndicated column in my new venture: the Laboratory Shopping Network! As an investor, you could ensure your own wealth by promoting our products in your column. Tis month, we will have a special on zircon knives for microscopists who cannot afford diamonds. Grayson in Hoosier Land
Dearest Grayson! Is this a trick question? I am already a millionaire! As for my extensive royalties, they are tied up in nanotech personal hygiene products. As for promotion, I already have several products that we developed and I am spokesperson for: a device to convert old glass plate lantern slides into JPEGs, 24K tennis bracelets made from spent sputter coater targets, a combination microtome/back massager, and my personal favorite, the snuggie lab coat! I would have more creations for product development, but I invested early on with Sir Tommy Sopwith and his “Bat Boat” and lost quite the bundle. My assistant assures me that you are an excellent risk, but I’ve learned to consult my financial advisor, Herr Bankrotteur, in all investment decisions. I’ll have my people contact your people.
If you have a bundle to invest, send your cash in unmarked bills to Herr Abbe in care of his assistant, Herr Shields at jpshield@
uga.edu.
doi:10.1017/S1551929511000605 79
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