Target Engagement in Live Cells
• Directly Measure Ligand Affi nity and Compound Residence Time
• Use Full-Length Protein • Assay Kinases, HDACS, and BET BRDs
Compound residence time measurement using NanoLuc®
-BRD4 Luciferase Fusion Protein. Tracer only
1,000nM JQ1 with washout 20,000nM JQ1, no washout
40 30 20 10 0 0 20 40 60 Time (minutes)
80
100
120
Direct residence time measurement of JQ1 at binding site on NanoLuc®
-BRD4 Fusion Protein.
Learn more about NanoBRET™ Target Engagement Assays at:
promega.com/ResidenceTime
©2017 Promega Corporation. All rights reserved. 37763493
BRET Ratio (mBU)
13725MA
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