ultra


ultra sonic Handling


Sample preparation • Trim the sample with a trim 45 or a trim 20 diamond blade. The sample width should be a maximum of 0.5 mm.


Measuring the sample height With the use of an eyepiece graticule in one of the stereomicroscope oculars (graticule 10450336 for the Leica M80). Sample block (fixed in the sample holder), is mounted in the trimming plate. Measure the height with the graticule.


Ultramicrotome Settings • Set the clearance angle to 6° as shown on the guarantee card.


• Set the desired section thickness. • Set the sectioning speed (0.4 – 0.6 mm/sec) • Tighten all set screws.


Installing the knife • Mount the knife in the knifestage of the ultramicrotome and tighten the set screw.


• Connect the control unit to the power supply. • Connect the control unit OUTPUT and the knife with the blue cable.


• Switch on the control unit (switch ON the back side). • Set the resonance frequency (Toggle switch on AUTO).After a few seconds the display will show «Peak locked».


• Adjust the amplitude to approx. 2 V. • Approach the sample with the knife (settings as shown in our diamond knife handling manual. • Start sectioning as usual.


Measuring the section length • Measure the section length with the graticule and compare it with the sample height.


• If sections are too short, increase the the amplitude (turn the button clockwise).


• If sections are too long, decrease the amplitude (turn the button counterclock wise).


• Too high an amplitude may lead to drifting of the sections. Drifting of the sections


When working in resonance, the sections may drift slightly to the right or to the left. If this is the case, the following procedure helps: Switching from AUTO to MAN. Now increase or decrease the the frequency a few hundred Hertz, until the sections float straight on the water surface.


If sections drift to the right: decrease the frequency. If sections drift to the left: increase the frequency.


9


Control Unit Frontside:


AMPLITUDE OUTPUT


DiATOME V7 R0.1 2.0V


35.0kHz


FREQUENCY AUTO


MAN


Output:


Amplitude: Frequency:


Connection with the knife (blue cable) 0 – 25.5 V


Toggle switch MAN: Manual setting of frequency, range 15 – 35kHz Toggle switch AUTO: Automatic setting of the resonance frequency.


Backside: Diatome sonic V7


SERIAL: 7210 SWISS MADE


The device complies with part 15 of FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) This device must accept any interference received, including interference that may cause undesired operation.


OFF


ON


9-15V 1A


ON/OFF Switch Connection for the power supply adapter.


Specifications Knife:


Knife angle: Cutting range:


ultra sonic, ultra sonic Maxi 35°


20 – 100 nm


Cutting edge length: 3.0mm, 4.0mm Order number:


Control unit Frequency range: Amplitude: Mains voltage:


15 – 35kHz, or automatic setting of the resonance frequency variable (voltage 0 – 25.5V) 230 V, 110 V


DUS3530 (for ultra sonic) DUSM3530 (for ultra sonic Maxi)


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32