cryo 25°


The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least pos- sible compression and the best structure preservation (H.M. Han et al., Journal of Microscopy, Vol. 230, Pt. 2, pp. 167 – 171, 2007).


Please note: best results are achieved at low humidity, when the cryo-ultrami- crotome is placed in a glovebox and the sections attached by electrostatic force (J. Pierson et al., Journal of Structural Biology 169, pp. 219 – 225, 2010).


High resolution electron micrograph of vitreous section of keratin intermediate


fi laments in the midportion of stratum corneum of human epidermis. The fi ne structure of the keratin fi laments is well resolved and their molecular organisation is seen in favourable cases (inset). Scale bar = 100 nm. Scale bar inset = 20 nm. Ashraf Al-Amoudi, Laboratoire d‘Analyse Ultrastructurale, Lausanne.


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cryo


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