cryo immuno 500 nm


The first cryo knife with a diamond platform, guarantees the best possible sectioning for sucrose infiltrated samples (Tokuyasu).


The diamond platform guarantees an easy and gentle section pick-up.


The sections are collected directly from the diamond surface using a loop and a sucrose/methyl-cellulose droplet (W. Liou et al., Histochemistry and Cell Biology, Vol. 106, pp. 41 – 55, 1996. P. J. Peters et al., Current Protocols in Cell Biology, pp. 4.7.1 – 4.7.18, 2006).


The 35° angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preserva- tion in sucrose infiltrated samples (E. Bos et al., Journal of Structural Biology 175, pp. 62 – 72, 2011).


We recommend the cryo immuno knife also for sectioning frozen hydrated samples (CEMOVIS). The 35° angle is a good compromise between durability and cutting performance (A. Al-Amoudi et al., Journal of Structural Biology 150, pp. 109 – 121, 2005).


Mouse optic nerve, immunolabeling of the major myelin protein proteolipid protein (PLP), 10 nm gold. Wiebke Möbius, Dept. of Neurogenetics, EM Core Facility, MPI of Experimental Medicine, Göttingen.


Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti- CD63 and protein-A gold 10nm. Scale bar = 100nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam.


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