ultra sonic
• Thinner sections • No compression • Best structure preservation
The patented ultra sonic knife allows the cutting of ultrathin sections free of compression (D. Studer et al., Journal of Microscopy, Vol. 197, pp. 94 – 100, 2000).
With correct setting of frequency and amplitude the sections become as long as the height of the sample.
Best results are not only achieved with biological samples, but also with poly- mers (J. S. Vastenhout et al., Microscopy and Microanalysis 8, 2001. J. S. Vasten
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hout et al., Microscopy Today, pp. 20 – 21, 2006).
We have tested the ultra sonic knife with the following samples: • Biological samples in Epon, Araldite, EM Bed, etc.
• Biological samples in acrylic resins (Lowicryl, LR White).
• Rigid polymers such as PS, PMMA, ABS, HIPS, modified PP, blends of various kind.
Polycarbonate modified with rubber Jens Sicking, Bayer Technology Services, Leverkusen.
200 nm
Peripheral nerve (rat), HP frozen, freeze substituted, Epon embedded, cut with the ultra sonic knife, section thickness 50nm. W. Graber, Institut of Anatomy, University of Bern, Switzerland
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