Serial sections obtained with the ultra sonic Maxi knife at 55 nm from a high pressure frozen nematode sample. A) Long ribbons collected on silicon wafers imaged using a SEM B) Gallery of serial sections C) Single section from the series. Scale bar 10 µm D) Yolk granules and lysosomes were segmented shown in green. Scale bar 5 µm; detail image: 1 µm Center for Microscopy and Image Analysis, University of Zürich, Switzerland