Examples of Staining PCNA (Nuclear antigen)


Sections of formalin-fixed, paraffin embedded tissue of human duodenum were deparaffinized and processed in Retriever to unmask the antigen. We used one cycle in R-Buffer C (pH 6.0) with cooling of the slides overnight. Antibody PC-10 against proliferation marker PCNA (nuclear) was used together with R-Detect HRP detection system for immunostaining of sections.


E-cadherin (Membranous, extracellular domain)


Sections of formalin-fixed, paraffin embedded tissue of human cervix were deparaffinized and processed in Retriever to unmask the antigen. We used one cycle in R-Buffer C (pH 6.0) with cooling of the slides overnight. Antibody HECD-1 against cell adhesion molecule E-cadherin was used together with R-Detect HRP detection system for immunostaining of sections.


Ki-67 (Nuclear antigen)


Sections of formalin-fixed, paraffin embedded tissue of human sigmoid were deparaffinized and processed in Retriever to unmask the antigen. We used one cycle in R-Buffer C (pH 6.0) with cooling of the slides overnight. Antibody MIB-1 against proliferation marker Ki-67 (nuclear) was used together with R-Detect HRP detection system for immunostaining of sections.


CD8 (Membrane)


Sections of formalin-fixed, paraffin embedded tissue of human sigmoid were deparaffinized and processed in Retriever to unmask the antigen. We used one cycle in R-Buffer A (pH 8.1) with cooling of the slides overnight. Antibody #4B11 against T-cell marker CD8 (cell membrane) was used together with R-Detect HRP detection system for immunostaining of sections.


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Retriever Tissue Slides


Retriever Tissue Slides are high adhesive glass microscope slides for paraffin- embedded tissue section, including tissue arrays. The slides were designed and optimized for the EMS Antigen Retriever (62700 series) to preserve the section attachment and tissue morphology during heat-induced epitope recovery in Retriever in pH6 (Low), pH9 (High), or Universal Retriever buffer.


Slides can also be successfully used in any other epitope-recovery technology (autoclave, microwave, proteolitic) and show superior performance over positively charged or polylysine-coated slides, especially in high pH buffers and recovery buffers containing EDTA.


These slides are ideal for both IHC and immunofluorescent applications, as well as for all antigen recovery systems.


Each of the slides have a custom imprinted frosted colored end, each color representing a different glass surface.


General Properties


z Retriever Tissue Slides are made from soda lime glass z Dimensions approximately 76 x 26mm, Thickness 1.0mm. z 90° ground edges z Colored or white marking area 20mm, at one end, on one side z Pre-cleaned, ready for use z Autoclavable z Supplied in plastic boxes of 100 pieces z Certified for in-vitro-diagnostic (IVD) application


HistoBond® + F, Blue


Both surfaces of these slides bond tissue sections adhesively with their positive charge. No additional adhesives are required. The reliable anchoring prevents tissue sections to float during washing and cooking. Reliable for routine IHC with FF/PE sections that involve antigen retrieval.


Cat. No. 71880-50 Description Retriever Tissue Slides, HistoBond® 71880-50-CS Retriever Tissue Slides, HistoBond® HistoBond® + R, Orange


Superior type of adhesion slide with a considerably higher positive charge than other regular charged slides. This results in strong adhesion of the tissue, even under alkaline conditions of EDTA/EGTA- containing buffers. IHC, IF, in-situ, hybridization, grade slides.


Cat. No. 71890-50 Description Retriever Tissue Slides, HistoBond® 71890-50-CS Retriever Tissue Slides, HistoBond® HistoBond® + X, White


Tissue sections anchor covalently on the glass surface. Even a non-polar tissue, e.g. very fatty (breast, brain, etc.) that does not cause a charge difference between the adhesive layer and the section, reliably bonds chemically to the glass.


Cat. No. 71890-51 Description Retriever Tissue Slides, HistoBond® 71890-51-CS Retriever Tissue Slides, HistoBond®


+ X, White + X, White


Qty. 100/pk 20 pk/cs


+ R, Orange + R, Orange


Qty. 100/pk 20 pk/cs


+ F, Blue + F, Blue


Qty. 100/pk 20 pk/cs


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