Retriever 2100 for Antigen Unmasking


Choosing A Buffer


If you already know the buffer that can be used for Microwave treatments of sections in order to unmask your antigen of interest, chances are high that the same buffer may be used in the Retriever.


To improve the morphology of tissue of the processed sections, use one of our supplied ready-to-use buffers by choosing them according to pH.


We have commercially available the following buffers:


z R-Buffer A pH 6.0 z R-Buffer B pH 8.0 z R-Buffer C pH 4.5


z R-Buffer U pH 6.0


z G variants of the same buffers for a more gentle processing of the tissues.


To successfully retrieve the antigen of interest on fixed sections, please remember that two factors define the choice of buffer:


z The nature of the antigen/epitope and of the antibody used for its detection


z The fixative used and the degree of fixation. Correction for Fixative & Degree of Fixation


The suggested protocol for processing tissues is optimized for routinely formalin-fixed and paraffin embedded material. If the tissue used for sections was insufficiently fixed, overfixed (was left in formalin for too long), or another fixative was used, the protocol may require some modifications.


The easiest correction is to use EMS's own specially formulated buffers for a gentle (G) tissue processing. Use them instead of the basic buffers. For overfixed material try using U buffer or run the additional cycle in the same buffer.


Fixative Used on Tissues Formalin, buffered Formalin-Zn


Mirsky fixative Boonfix I Boonfix II PLP


Buffer


R-Buffer A, B, C or U R-Buffer A, B, C or U Under testing Under testing Under testing


R-buffer AG, BG, or CG Correction for the Nature of the Antigen and Epitope.


The choice of the buffer depends on the nature of the antigen and the location of the epitope of interest. We advise you to first run the test for the most appropriate buffer using the tissues where the expression of the antigen does occur. General guidelines for choosing the buffer:


Most of the nuclear antigens (apoptosis-related, survival-related, proliferation-related) R: Buffer A (or AG).


Cell adhesion molecules, cell membrane antigens (extracellular domain) R: Buffer A (or AG)


Cytoskeleton and cytoskeleton-associated molecules – R: Buffer A (or AG)


Intracellular domain of some adhesion molecules and surface receptors – R: Buffer B (or BG)


Intracellular domain of some adhesion molecules and surface receptors – R: Buffer C (or CG)


Most of antibodies raised against a linear peptide – R: Buffer U (or UG)


Specifications Height


Capacity


Max. Instrument length Width


Net Weight


Internal Chamber Dimensions (d/h) Max. Load Weight


335 mm 9 liters


228 mm 340 mm 4.5 kilos


210/230 mm 3.0kg


Fuses — Located under the control module, fuses F1 OA, 32 x 6.3mm, ceramic sand filled, Mains plug top fuse (User replaceable), F1 3A to BS1362 UK ONLY.


Rating — Models are rated continuously for intermittent use. Body — Deep drawn aluminum. Lid - Aluminum.


Heater — Externally surface mounted mechanically fixed electric element.


Temperature Cut Out — Thermal fuse. Pressure — Calibrated pressure release valve. Max. Single Fault Temperature — 133.3°C


Environment Conditions — indoor use - temperature 5°C to 40°C - altitude up to 2000m - maximum relative humidity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C. - mains supply voltage fluctuations not to exceed +10% of the nominal voltage


Input Connections — Mains inlet socket 'hot' format conforming to IEC 302.


Safety Shut Down — See "Temperature Cut Out".


Ordering Information Cat. No.


Description


Retriever 2100 62700-20 62700-10


Retriever 220 volt Retriever 115 volt


Buffers and Accessories 62706-10 62706-11 62706-12 62706-13 62707-10 62707-11 62707-12 62707-13 62705-01 62705-02 62705-03 62705-04 62705-05 62705-06 62705-07


R-Buffer A (10x) R-Buffer B (10x) R-Buffer C (10x) R-Buffer U (10x) R-Buffer AG (2x) R-Buffer BG (2x) R-Buffer CG (2x) R-Buffer UG (2x) Slide Chamber Chamber Rack Lifting Device Cord Set (UK)


Cord Set (Europe) Cord Set (US)


Green Silicone Sealing Gasket ImmunoSaver Antigen Retriever


Immunosaver allows for Immunostaining with quick and easy activation of cell membranes and the nucleus. Immunosaver provides efficient antigen retrieval for successful immunostaining of a wide variety of antigens under optimized conditions.


Protocols for both Light and Electron Microscopy may be found with this reagent.


Cat. No. 64142


Description ImmunoSaver


Qty. 100 ml 3


Qty.


each each


250 ml 250 ml 250 ml 250 ml 250 ml 250 ml 250 ml 250 ml 3/pk each each each each each each


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