Retriever 2100 for Antigen Unmasking Epitope Recovery Buffers


Nevertheless, we found some cases when buffers besides R-Universal achieve a better result. Depending on the structure of the epitope and amino acids comprising it, different buffers should be used for epitope recovery.


The type of epitope defines the most appropriate recovery buffer


In cases where R-Universal may not be the best buffer, as well as standard EDTA etc, the charts below may be used to choose the most appropriate buffer for epitope recovery in relation to the type of the epitope.


In total we offer 5 different buffers for antigen recovery. Most epitopes can be perfectly recovered in R-Universal buffer, including those usually recovered in Citrate/EDTA/Tris-EDTA buffers. Two new buffers offer solutions for rare, but still existing cases when antibodies give an incorrect pattern on a retrieved section or no staining at all. We also now offer analogs of standard Low ph (Citrate) or “L-Recovery” and High ph “H-Recovery” (EDTA) buffers in case you already use antibodies known to work after tissue processing in one of these buffers.


Ordering Information Cat. No.


Description


4˚C 4˚C 4˚C 4˚C 4˚C 4˚C 4˚C 4˚C 4˚C 4˚C


62719-10 62719-20 62719-30 62719-35 62719-40 62719-45 62719-50 62719-55 62719-60 62719-65


R-Universal Epitope Recovery Buffer (10x stock) R-Universal Epitope Recovery Buffer (10x stock) T-Universal Epitope Recovery Buffer (10x stock) T-Universal Epitope Recovery Buffer (10x stock) U-Recovery Epitope Recovery Buffer (10x stock) U-Recovery Epitope Recovery Buffer (10x stock) L-Recovery Epitope Recovery Buffer (10x stock) L-Recovery Epitope Recovery Buffer (10x stock) H-Recovery Epitope Recovery Buffer (10x stock) H-Recovery Epitope Recovery Buffer (10x stock)


HEIR Buffer Selection Chart Antibody raised against:


Hydrophilic peptide epitope or bacterially produced


fragments of a target protein


Yes No


Qty.


125 ml 500 ml 125 ml 500 ml 125 ml 500 ml 125 ml 500 ml 125 ml 500 ml


Epitope Recovery Optimization Buffer Set


2100 Retriever allows the use of up to 6 different buffers in one cycle, each of the Slide Chambers accommodating one buffer. Each individual Slide Chamber can accommodate up to 18 slides. You can optimize the recovery buffer (and/or test the usability of buffer for recovery) for 18 individual antibodies in one go.


Such runs allow the establishing of the best/acceptable epitope recovery conditions for all antibodies that can later be used in experiments, which is especially important if you plan an IF multicolor staining of tissue sections.


We advise to do the primary test for individual antibodies with immunoenzyme staining, as this allows for better analysis of the pattern of staining and (eventual) non-specific staining of some areas/structures in section (i.e. blood vessels etc.)


For your convenience, we offer a complete set of different recovery buffers for the tests, containing 125 ml of each 10x buffer stock.


Cat. No. 4˚C 62719-70 Description Qty.


Epitope Recovery Buffer Set, includes one each 5 x 125 ml of the above Recovery Buffers (125 ml)


Unless the specificity of such antibody can be verified by immunoprecipitation it’s better to look for another one.


Western Blot: Non–reduced Western Blot: Reduced


Yes No


Appropriate Buffer:


R–Universal R–Universal


Isolated native antigen or


complex of native proteins, cells, whole viruses, bacteria


Yes No


Yes No


R–Universal


R–Universal T–Universal


Hydrophobic or neutral peptide epitope, or


denaturated native protein


Yes No


Yes No


U–Recovery R–Universal


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