Reduce The Background, Not The Specific Staining Antibody Diluent


Buffer for diluting your primary and secondary antibodies, especially if they were stored for a while, even at -20 in glycerol, or in refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimized, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.


Reliable time tested solution to unmask antigen on formalin-fixed sections


Any antibody preparation has some potential to produce non-specific reaction in the assay. This originates from:


• non-specific antibodies that are present in some proportion in any polyclonal antibody preparation, including affinity purified ones (often “affinity purified” means only isolation of IgG fraction on Prot A/G column, not the purification on the antigen column under very stringent conditions)


• low specificity antibodies among specific ones in polyclonal • fragments of fallen apart IgGs in stored preparations, including monoclonal


• separate heavy and light chains of specific antibodies, produced by most hybridomas


All these are capable of binding non-specifically to molecules on tissue sections, blots, fixed cells and other objects for immune detection. In the case of retrieved formalin sections the risk of non-specific reaction is even more, since to activate the epitope recovery the proteins comprising the tissue sections are denatured during HIER, thus making accessible many domains that are charged and are capable of binding the test immunoglobulins on non-specific manner.


The standard means to block non-specific binding of specific antibody preparation is to add some irrelevant protein, such as BSA, other serum, casein, etc. However, everyone who has tried to do this knows that increasing (for effective blocking) concentration of such blocking agent leads to a great reduction of specific reaction as well. This is due to large blocking molecules binding to accessible sites on section and thus sterically blocking access of specific antibodies to epitopes of interest (schematically represented in the figure on the left, top). All our buffers developed for immune assays contain instead short (0.6-2 kD) peptides that are capable of effectively blocking non-specific reaction while not affecting the specific binding of antibody.


The presented collection of Immunohistology buffers also has some other benefits (see page 7) that allow you to achieve the best quality IHC result without compromising the antigen detection. The buffers can also be used in other immune assays, such as immunofluorescence on sections, flow cytometry on fixed cells, western blot, hybridization of sections with antibody detection.


The Retriever IHC Buffers empower you to control non-specific staining at every step of immunohistochemistry. They are especially highly recommended for research pathology where, in contrast to diagnostics, many polyclonal and/or low-affinity antibodies are used.


All buffers are available in 50 ml, 125 ml and 500 ml quantities and are ready to use.


When used in pathology, in also greatly reduces non- specific reactivity of human serum components and immunoglobulins in tissue, vessels and cells with mouse antibodies used on section.


For especially "trouble"-giving antibodies, as well as for in situ PCR applications, this dilutent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.


Cat. No. 62713 62714 62715


62713-01 62714-01 62715-01


Description


Antibody Dilutent Antibody Dilutent Antibody Dilutent


Antibody Dilutent for Frozen Sections Antibody Dilutent for Frozen Sections Antibody Dilutent for Frozen Sections


HRP-Conjugate Dilutent


Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay preparing ready-to-use conjugate solutions in advance and store them in refrigerator without loss of activity.


Cat. No. 62716 62717 62718


62718-15 Description


HRP Conjugate Dilutent HRP Conjugate Dilutent HRP Conjugate Dilutent


Slide Washing Buffer 10 X Cat. No.


Description Slide Washing Buffer 10 X Section Block


A new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section. Greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum-based blocking solutions, there is no interaction of specific antibody and blocking protein itself. Therefore, it is not comparable to other commercially available or home-made blocking solutions. Recommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.


Cat. No. 62710 62711 62712


6


Description Section Block Section Block Section Block


Qty. 50 ml


125 ml 500 ml


Qty.


50 ml 125ml 500 ml


Qty. 500 ml Qty. 50ml


125ml 500ml 50ml


125ml 500ml


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