EMS Antigen Retrievers in IHC I. Trypsin Reagent


Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer. Storage: 2–8˚C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes. Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml. Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml Cat. No.


Description


64142-01 62142-02


Trypsin Kit Small Trypsin Kit Large


II. Pronase Reagent, Ready To Use


Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: Ready-to-use Pronase reagent available in 2 sizes: 15 and 100 ml.


Cat. No.


64142-03 62142-04


Description


Pronase Reagent Pronase Reagent


III. Pepsin Reagent, Ready To Use


Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 ml


Cat. No.


64142-05 62142-06


Description


Pepsin Reagent Pepsin Reagent


IV. Citrate Buffer pH 6.0 (10x)


This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: 1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10 X, pH 6 antigen retriever solution.


Qty. 15 ml 100 ml


VII. ImmunoHistoZyme™, Ready-to-Use ImmunoHistoZyme is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: Ready-to-use ImmunoHistoZyme Reagent.


Cat. No.


64142-13 62142-14


Description


ImmunoHistoZyme ImmunoHistoZyme


Qty. 15 ml 100 ml 7 Qty. 15 ml 100 ml


Qty. Kit Kit


Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2–8ºC


Cat. No.


64142-07 62142-08


62142-08-1G Description


Citrate Buffer pH 6.0 Citrate Buffer pH 6.0 Citrate Buffer pH 6.0


V. EDTA Buffer pH 8.5 (10x)


This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: 1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10 X, pH 8.5 antigen retriever solution. Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2-8ºC


Cat. No.


64142-09 62142-10


62142-10-1G Description


EDTA Buffer pH 8.5 EDTA Buffer pH 8.50 EDTA Buffer pH 8.50


VI. Tris Buffer pH 10.0 (10x)


This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2–8˚C. DO NOT FREEZE Intended Use: 1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10 X, pH 10 antigen retriever solution. Preparation of Working Solution: Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2-8ºC


Cat. No.


64142-11 62142-12


62142-12-1G Description


Tris Buffer pH 10.0 Tris Buffer pH 10.0 Tris Buffer pH 10.0


Qty. 100 ml


1000 ml 1 Gal.


Qty. 100 ml


1000 ml 1 Gal.


Qty. 100 ml


1000 ml 1 Gal.


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