BIOTECH & LIFE SCIENCES Average Recovery (%RSD)
Analytes 13C-TFA PFPrA PFBA
PFPeA PFHxA PFHpA PFOA PFNA PFDA TFMS PFEtS PFPrS PFBS
PFPeS PFHxS PFHpS PFOS PFNS PFDS
HFPO-DA ADONA
Linearity Range LOD (ng/mL) 0.50 - 40 0.25 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.05 - 40 0.05 - 40 0.05 - 40 0.05 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.10 - 40 0.50 - 40 0.10 - 40
9Cl-PF3ONS 0.10 - 40 11Cl-PF3OUdS 0.10 - 40
nominal value. The average measured concentrations of most PFAS in the SRMs closely matched the reference concentrations and, when calculated as relative values, were within 20%. While the measured relative concentration of PFDA in NIST SRM 1957 had a slightly higher deviation (26%), it remained within the SRM’s reference concentration deviation range. These results demonstrated that the method is suitable for accurate measurement of PFAS in both human plasma and serum samples.
CONCLUSION The simple, reliable workflow established here incorporates ultrashort-chain PFAS into a method
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(ng/mL) 0.1250* 0.0102 0.0222 0.0125 0.0098 0.0050 0.0051 0.0012 0.0083 0.0070 0.0020 0.0030 0.0124 0.0031 0.0115
0.0088 0.0200 0.0129 0.0111 0.1875 0.0035 0.0031 0.0023
Fortified Concentration (ng/mL) 0.4 –
2
108 (2.53) 104 (5.57) 97.4 (3.25) 99.2 (2.46) 86.7 (5.37) 94.8 (8.24) 96.2 (1.44) 93.6 (1.93) 89.4 (7.33) 98.0 (2.62) 98.1 (8.18) 88.0 (8.84) 94.8 (4.57) 85.8 (7.88) 92.5 (6.75) 97.8 (8.66) 92.1 (7.98) 92.6 (8.20) –
90.4 (7.14) 95.8 (4.20) 106 (5.10)
90.6 (9.28) 115 (0.965) 109 (1.75) 93.3 (4.19) 109 (4.65) 99.2 (2.04) 107 (5.30) 111 (1.87) 102 (2.03) 88.8 (3.80) 103 (1.47) 108 (4.23) 94.1 (4.95) 100 (8.05) 96.0 (10.0) 99.8 (6.45) 97.9 (7.01) 94.2 (4.43) 82.3 (3.48) 99.9 (11.3) 106 (4.67) 93.7 (5.12) 84.5 (4.20)
10
98.0 (2.99) 105 (6.99) 104 (1.51) 89.1 (5.57) 102 (6.31) 89.2 (1.80) 95.6 (3.35) 99.1 (2.62) 95.5 (3.64) 91.9 (3.19) 99.3 (2.26) 98.9 (4.15) 86.5 (6.17) 94.3 (5.43) 92.2 (8.98) 93.4 (5.74) 95.9 (3.37) 91.9 (2.78) 87.6 (3.55) 91.1 (8.95) 95.7 (4.25) 93.8 (6.71) 97.2 (4.86)
Table I: Linearity, LOD, accuracy, and precision for fortified FBS samples (n = 9, interday) *for non-labelled TFA It uses a polar-
embedded Ultra IBD column to increase retention of these small polar analytes, and the results demonstrate that the method is accurate and precise
that also includes longer-chain PFAS that are commonly monitored in human plasma and serum. It uses a polar-embedded Ultra IBD column to increase chromatographic retention of these small polar analytes, and the results demonstrate that the method is accurate and precise. Most important, it can be a valuable tool for studying human exposure to emergent ultrashort-chain PFAS. Shun-Hsin Liang PhD is a senior
principal scientist for Restek Corporation
For more information visit:
www.restek.com
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100 (10.7) – – – – – – – – – – – – – – – – – –
90.4 (6.13) – – –
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