BIOTECH & LIFE SCIENCES
HOLOTOMOGRAPHIC IMAGING EXPLAINED The product also uses Tomocube’s holotomographic imaging solution involving the illumination of a live cell with low power visible light from multiple illumination angles, this is followed by measurement of the phase delay of the transmitted light. The technique uses the measured refractive index as imaging contrast, allowing visualisation of 3D living cells and tissues without labelling or staining. With its high spatial resolution capable of capturing subcellular organelles, users have increasingly sought confocal fluorescence capabilities to design multiplex imaging workflows.
SDCM technology helps researchers perform challenging live imaging experiments
SPINNING DISK CONFOCAL TECHNOLOGY CrestOptics is a specialist in spinning disc confocal technology (SDCM). This solution represents an alternative to laser scanning confocal technology (LSCM) and typically affords more detailed images. Rather than a single pinhole, a SDCM has hundreds of pinholes arranged in spirals on an opaque disk which rotates at high speeds. When spun, the pinholes scan across the sample
in rows, building up an image. The product will help researchers
routinely perform challenging live-imaging experiments for extended periods of time. The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the spinning disc confocal tool a standard for 3D live cell imaging, according to the company.
WHAT THE COLLBORATION WILL MEAN FOR CUSTOMERS By combining these two non-invasive imaging modalities, the HT-X1 Plus affords more complete insights into molecular distribution within samples. Additionally, the integrated platform provides precision alignment of HT and fluorescence-based imaging datasets, allowing researchers to easily study molecular and phenotypic data in tandem and generate more powerful insights across biophysical imaging applications. Dr Alessandra Scarpellini, chief
commercial officer, CrestOptics, says “The integrated platform offers more complete insights into the molecular and phenotypic data from 3D cells and tissues, without requiring invasive methods, labelling or staining.” Dr Sumin Lee, VP of customer
development at Tomocube, said: “Collaborating with CrestOptics marks a major milestone in our commitment to meeting the needs of our customers. We’ve seen a growing demand for a solution that combines the label-free imaging of holotomography with the precision of fluorescence-based microscopy. The HT-X1 Plus provides unprecedented resolution and sensitivity for 3D biophysical imaging applications.”
For more information visit:
https://crestoptics.com and
https://www.tomocube.com
Dr Alessandra Scarpellini Dr Sumin Lee
www.scientistlive.com 35
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