BIOTECH & LIFE SCIENCES


Figure 1: Analysis of a 10 ppb PFAS Standard 11Cl-PF3OUdS


All analytes exhibited


recovery values within the range of 82.3 – 115% across the three fortification levels


9Cl-PF3ONS ADONA


-0.001.002.003.004.005.006.007.008.00


(C6) PFHxA


(C2) PFEtS


(C1) TFMS


(C3) PFPrA


(C2) TFA


-0.00 TFA 1.00 2.00 3.00 4.00 PFHxS 5.00 Time (min) PFOS 6.00 7.00 8.00 9.0010.00


(C4) PFBA


(C3) PFPrS


(C4) PFBS


(C5) (C5) (C6) (C7) (C9) (C8) (C10)


(C7) PFHpA


(C10) PFDA


(C8) PFOA


9.0010.00


(C9) PFNA


2.80


3.00


3.20


3.40


3.60


3.80 6.90 7.00 7.10


7.20 7.30 7.40 7.50 7.20


7.40


7.60


7.80


8.00


8.20


RESULTS AND DISCUSSION As shown in Figure 1, strong retention and good separation of early eluting, ultrashort-chain PFAS were obtained under reversed-phase conditions using the polar embedded Ultra IBD column. More important, the extensive retention of the ultrashort-chain PFAS, especially for the first-eluted analyte (TFA), led to reduced matrix interferences.


Table I shows the specific linear


range for each analyte, and all analytes had acceptable linearities (r2


>0.995) and deviations (<20%).


Accuracy and precision for most analytes were assessed at 0.4, 2, and 10 ppb. However, HFPO-DA and TFA were evaluated at 2, 10, and 30 ppb because 0.4 ppb was below their linear range. All analytes exhibited recovery values within the range of 82.3–115%


across the three fortification levels. Satisfactory method precision was demonstrated with %RSD values of 0.965–11.3%. The LOQ was set as the lowest calibration concentration for each analyte, and the LOD was defined by a peak signal-to-noise ratio of 3:1 in fortified FBS. Six preparations of each SRM


were analysed and all measured EIS concentrations fell within 20% of the


www.scientistlive.com 37


PFPeA PFPeS PFHxS


PFHpS PFOS


PFNS PFDS HFPO-DA


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52