MICROSOCOPY & IMAGING
STORIES I
SPATIAL TISSUE SCYTOMETRY
UCCESS
Anastasiia Marchuk and Felicitas Mungenast present applications of spatial tissue cytometry
n-depth phenotyping within tissue sections is gaining more importance in research and clinics. It is no longer enough to perform H&E or single-
marker staining but to capture and analyse the complexity of multiple cellular sub-populations, including their spatial context in the tissue microenvironment. Tese evolving needs present many challenges, such as multiplexed imaging due to the high number of stained markers, quantification of multiple markers to determine tissue structures and cellular and molecular phenotypes, as well as high-content data mining. Tese and other challenges raise questions about appropriate equipment, which should be both precise enough to generate reliable data and able to process the samples as quickly as possible. Tis comes together with the emerging
crisis of reproducibility in science, which is an obstacle to the correct interpretation of research and thus research in general.[1]
For these
reasons scientists/clinicians look for solutions that allow for the standardisation of their studies and reducing the prevailing bias. In the case of image processing, slide-based cytometry and computational pathology, this can be achieved by using a more robust and observer independent methodology.
Tissue cytometry is used for a variety of research applications
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HIGH-END TISSUE CYTOMETRY IN CANCER RESEARCH Te basic process of conducting cellular phenotyping involves the preparation and staining of samples for multiple markers. Classically, flow cytometry is used to gain information on expression patterns and the percentage of cells expressing certain markers, but it does not offer any data on the spatial distribution of cellular subpopulations. Tissue cytometry, however, allows the reaching of several aims: gene and biomarker expression patterns; cell population locations; and spatial interactions and cell dynamics within the native tissue
TissueFaxs cytometers are highly flexible imaging and analysis platforms for IF/IHC processed samples
microenvironment. Tese questions are particularly important in the fields of immunology and oncology. TissueGnostics’ (TG) tissue cytometry solutions, including the TissueFAXS platform (modular slide imaging solution) and StrataQuest (contextual imaging analysis solution), have been used in hundreds of projects covering cutting- edge topics within cancer research. For example, a recent paper showed that a distinct subpopulation of CD8+ T cells is present at higher levels in colorectal cancer with liver metastases relative to adjacent liver tissue.[2]
Tis subset of
CD8+ cells has now been evaluated in detail using the TissueFaxs Chroma
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