23 Proteomics, Genomics & Microarrays Conclusion
On comparison with low binding products from other vendors, serial incubation of a 1.1kb linear DNA fragment showed no signifi cant loss of DNA in Azenta Life Sciences low binding plates, while some loss was seen from low starting concentrations in plates made of alternative materials or competitor low binding branded plates, resulting in signifi cantly higher Ct values (up to 3 cycles). Additionally, incubation of mouse genomic DNA over a broad temperature range of 4°C, 37°C and 65°C had no signifi cant impact on DNA binding suggesting no loss of template during storage and PCR. Azenta Life Sciences low binding plates offer ideal properties for highly sensitive assays where low DNA input is vital and therefore any loss during transfer steps would have a signifi cant impact on the success of the assay. Low binding plastics offer advantages not only for sensitive techniques such as NGS but also for the storage of samples and use in the forensic market. Low binding products from Azenta Life Sciences offer reduced nucleic acid binding without the use of surface coatings that can introduce a source of leachables. This is achieved by carefully selecting PP polymers with low-
binding characteristics. Azenta is however working in partnership with Furtwangen University (Rottweil, Germany) and Plasma Electronics (Neuenburg, Germany) to investigate novel, chemical resistant coatings for plastic polymers to improve functional binding properties, without affecting samples.
References 1. Gaillard, C. and Strauss, F. (1998) Technical Tips Online Vol 3, 63-65. 2. Belotserkovskii, B. and Johnston, B. (1997) Analytical Chemistry 251, 251-262. 3. Shimizu, E et al. (2010) Shokuhin Eiseigaku Zasshi 51, 43-47.
Further information:
Steve Knight is Marketing Manager of the Consumables & Instruments Group at Azenta Life Sciences and may be contacted on
steve.knight@
azenta.com
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www.labmate-online.com/article Precision LC columns for advanced proteomic analysis
GL Sciences’ Bio LC Columns ‘ProteoSil’ are tailored HPLC solutions designed specifically for proteomics and the analysis of bio molecules such as proteins, peptides, and nucleic acids.
The packing materials used in these columns are crafted from high-purity silica, offering pore sizes of 100Å, 200Å, and 300Å. They are available in multiple configurations, including reversed phase, HILIC, and size exclusion columns. This versatility allows for a wide range of analytical possibilities.
One notable feature of the ProteoSil HPLC columns is their precise control over pore sizes in the packing material. The 200Å pore size is particularly optimised for the analysis of compounds with molecular weights ranging from a few kDa to several tens of kDa. This makes it perfect for the analysis of peptides and oligonucleotides, delivering exceptional performance in these applications.
Furthermore, users can choose between stainless steel and BioInert PEEK for the column hardware. The stainless-steel coated PEEK hardware design has enhanced the maximum operating pressure of the columns, providing greater flexibility in usage.
Quality and consistency are paramount, and GL Sciences ensures this by holding ISO 9001 and ISO 14001 certifications at their production facility. They oversee all processes, from matrix synthesis and chemical treatment to column packing and quality inspections, in-house. Their continuous evolution based on accumulated expertise guarantees the delivery of superior HPLC columns.
In addition to the mentioned features, GL Sciences offers chemical modification of functional groups, endcap processing, and the synthesis of silica gel, all critical components that contribute to exceptional column performance. With GL Sciences Bio LC Columns, researchers can rely on high-quality, globally accessible HPLC solutions for their bio molecule proteomics needs.
More information online:
ilmt.co/PL/bV5Z 61994pr@reply-direct.com Automated parallel capillary electrophoresis system simplifi es protein analysis
The Agilent ProteoAnalyzer system is an automated parallel capillary electrophoresis system for protein analysis. This innovative platform simplifies and enhances the efficiency of analysing complex protein mixtures, catering to diverse sectors including pharma, biotech, food analysis, and academia.
Capillary electrophoresis (CE) has become a cornerstone in protein separation due to its rapid, high-resolution analysis and minimal sample consumption. With growing interest in monoclonal antibodies and therapeutic protein targets, the demand for CE solutions is on the rise.
The ProteoAnalyzer automates capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) analysis of reduced and non-reduced protein samples. Processing 12 samples in just 30 minutes, it eliminates the cumbersome SDS-PAGE gel preparation, staining/destaining, and analysis, allowing users to focus on results.
With the capability to separate proteins ranging from 10 to 240 kDa in a single run, the system accommodates diverse samples, including monoclonal antibodies, biosimilars, and crude lysates. Individual capillaries are rejuvenated between each separation, ensuring accurate analysis across various sample types.
The ProteoAnalyzer system enhances efficiency, versatility, and reliability, particularly in protein QC workflows. By automating separation, data processing, and simplifying sample preparation, it streamlines analysis workflows, reducing training and labour costs. Additionally, it can analyse different sizes and types of proteins in a single run, consistently delivering accurate results and minimising the need for reanalysis.
Knut Wintergerst, Vice President and General Manager of Agilent’s Biomolecular Analysis Division, emphasised the system’s efficiency, versatility, and reliability, stating that it simplifies the analysis of complex mixtures across diverse sectors, from pharma to academia.
As a market leader in CE, Agilent’s ProteoAnalyzer strengthens its position as a provider of quality control analysis solutions, extending its CE portfolio across relevant biomolecule classes and catering to the evolving needs of various industries.
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ilmt.co/PL/G75V 62526pr@reply-direct.com Highly consistent collagen fi bre scaffold for 3D cell culture
CollaFibR™ from AMSBIO is a collagen fibre matrix produced using patented dry-spinning technology. This innovative material closely mimics the biomechanical and biochemical properties of natural collagen scaffolds, providing a highly accurate representation of in-vivo conditions.
CollaFibR™ is manufactured on a commercial scale using automated Good Manufacturing Process technology, ensuring high consistency and quality. It caters to various applications in 3D cell culture and tissue engineering, offering physiologically relevant environments for cellular alignment, migration, and elongation.
The user-friendly 12-well plate insert format makes CollaFibR™ scaffolds ideal for both 2D and 3D cell culture applications. Custom scaffolds tailored to specific needs are also available, with control over thicknesses, porosities, and alignment, ensuring batch-to-batch consistency and high cell viability.
With options for fluorescent tagging, CollaFibR™ scaffolds are compatible with various microscopy techniques and come UV sterilised for immediate use. They are degradable with collagenase, facilitating easy cell extraction. Additionally, CollaFibR™ is offered as short dry-spun 50 μm collagen fibres, enhancing cellular viability, shape fidelity, and biological relevance in bioinks and hydrogels. CollaFibR™ scaffolds set a new standard for cell culture consistency, driving innovation in areas such as 3D tissue culture, myelination studies, and contract tissue engineering. More information online:
ilmt.co/PL/BXXR
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