Spotlight on PFAS - Environmental Laboratory 19 Legionella pneumophila test published as ASTM International Standard
ASTM International is an international standards organisation that develops and publishes standards applicable to a wide variety of industries. There are over 12,500 global ASTM standards, developed and reviewed by over 30,000 technical experts from 140 participating countries. Global water microbiology specialist, IDEXX, has announced that the Legiolert®
test, for the rapid detection of Legionella pneumophila, has been published by ASTM International as ‘ASTM D8429-21: Standard Test Method for Legionella pneumophila in Water Samples Using Legiolert’.
This latest standard inclusion follows Legiolert having been specifi ed as a recommended method in the by the UK’s Standing Committee of Analysts’ ‘blue book’ publication, ‘The determination of Legionella bacteria in waters and other environmental samples (2020) - Part 2 - Culture Methods for their detection and enumeration’. Legiolert is also recognised with AFNOR certifi cation for hot and cold sanitary water and cooling tower water (Reference No IDX 33/06 06/19).
A liquid culture method, Legiolert was launched in Europe and North America by IDEXX in 2017 as a highly sensitive method for the confi rmed detection of Legionella pneumophila (the primary cause of Legionnaires’ disease) in water, delivering results up to seven days faster than traditional ISO culture testing methods.
There are more than 10 peer-reviewed papers that have highlighted the superior performance of Legiolert when compared to the standard method for detection of Legionella pneumophila. The ISO 11731 method has a number of limitations including a signifi cant false negative error rate which can result in undetected contamination and consequent risk to human health. In contrast, Legiolert is a culture method which is both accurate and easy to use.
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Analysis of Polychlorinated Biphenyls (PCB) in environmental matrices by GC-MS/MS
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PCBs are resistant to decomposition in the environment, and tend to accumulate in living organisms. As a result, they are designated as POPs (Persistent Organic Pollutants). In monitoring these environmental pollutants in river water and sludge, sensitivity for the detection of trace quantities and separation from impurities in the samples become important issues. An application from Shimadzu demonstrates the analysis of 8 different PCB over a broad calibration range (0.1 μg/L to 200 μg/L). Instrument calibration was done using an internal standard approach. 8 levels between 0.1 μg/L and 200 μg/L have been measured for calibration and resulted in R2-value above 0.9995 for all compounds. Stability evaluation of the calibration was done using the internal standard (ISTD) stability. Each sample contained identical amount of ISTD spiked at the end of sample preparation. Excellent sensitivity combined with a high linear dynamic range was shown for standards and real world samples in PCB analysis using GCMSMS. Excellent sensitivity combined with a high linear dynamic range was shown for standards and real world samples in PCB analysis using GCMSMS.
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