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1 Contents Head Office ISSN: 1752-8070


International Labmate Ltd. Oak Court, Sandridge Park, Porters Wood, St Albans, Herts AL3 6PH, UK Tel: +44 (0)1727 855574 Fax: +44 (0)1727 841694 info@chromatographytoday.com www.chromatographytoday.com


Michael Pattison, Chairman International Labmate Ltd.


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Contributing Editors Alan Handley President of the Chromatographic Society alan.handley@lgc.co.uk


Dr John Lough


Reader in Pharmaceutical Analysis University of Sunderland john.lough@sunderland.ac.uk


Dr Paul Ferguson


Associate Principal Scientist, Astra Zeneca, Macclesfield


Dr Richard Houghton Principal Scientist


Bioanalytical Sciences, Quotient Bioresearch


Dr Tony Edge Technical Manager Thermo Fisher Scientific


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Published – February/March, May/June, August/September and November/December


Chromatography Today is distributed free of charge to scientists, laboratory technicians and environmentalists throughout the United Kingdom. The publication has the support of leading suppliers of equipment in the region and is displayed at the major exhibitions and conferences in these markets to attract additional readership. Chromatography Today is published with the full support of the Chromatographic Society.


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Published with the full support of the Chromatographic Society by International Labmate Ltd.


Printed in the UK by Printed by Warners Midlands PLC 56 Separation Science / Spectroscopy Meetings Calender 46


Why is my Method Not Robust? Chromatography Today Help Desk


38


Optimising Protein and Peptide Speed and Resolution with Superficially Porous Columns


32


Capillary Electrophoresis: an Attractive Technique for Chiral Separations


28


Controlling Selectivity on Zwitterionic HILIC Columns by Adjusting pH and Buffer Strength


17


Robust Separation of Polar Compounds Utilising Porous Graphitic Carbon (PGC)


2 Foreword by Alan Handley Articles 6


Hydrophilic Interaction Liquid Chromatography: an Investigation into the Solvent and Column Selectivity


22 How Good is SFC for Polar Analytes?


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