21


Norm. 120


Conclusion 100 80 60 40 20 0


Porous graphitic carbon columns can be used successfully for the retention and separation of extremely polar compounds, which are problematic to retain in reversed-phase conditions. These columns offer a unique polar retention mechanism that often can be implemented simply, and can produce robust separations of extremely polar species under typical reversed phase conditions. In the example presented the methodology was successfully validated and transferred to other laboratories.


0 5 10


Figure 7: Overlaid chromatograms showing consistency and method 2 robustness on Hypercarb 5 µm, 100 x 4.6 mm column.


This method was subsequently validated and transferred to five other laboratories where equivalent chromatography was successfully demonstrated.


min References


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[9] K. Gaudin, T. Hanai, P. Chaminade, A. Baillet, J. Chrom. A, 1157 (2007) 56-64


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[12] AstraZeneca Analytical Method Protocol


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