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Figure 5: SFC separation of nucleobases: 1. thymine, 2. uracil, 3. cytosine, 4. guanine and 5. adenine. Zorbax Rx-Sil, 1 mL/min ethanol-ammonium formate buffer-formic acid 20 mM pH 3 (95:5 v/v) and 2.0 mL/min CO2; 40°C. Reprinted with permission from [48]. Copyright 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
two major manufacturers of chromatographic devices, namely Waters and Agilent, will further the interest for this versatile and highly interesting technique.
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Table 4: Ionic compounds successfully analysed with packed column SFC
be used in the mobile phase (when compared to methanol). While the resulting fluid in such ternary compositions is not a supercritical fluid but a liquid with so-called ‘enhanced fluidity’, we have pointed out above that technically, the way to practice the technique is no different.
In summary, we hope we have proven here that analysis of polar compounds is not only the future, but also the past and present of SFC. Maybe SFC should not be simply viewed as a replacement for normal-phase and for non-aqueous reversed-phase HPLC, but also for HILIC methods. Now that the technique is fully mature, it would be good to re-visit some of the old studies. It is to be expected that the recent introduction of modern SFC systems by
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