ANALYSIS OF PESTICIDES, DYES AND DRUGS continued a
done in 15 minutes using the Obelisc R reversed-phase column. The ap- plication is LC/MS-compatible, and the low level of formic acid enhances ionization.2
As described, the method also preserves analytical sensitivity.
Within 8 minutes, the analyzer separated the active ingredients in com- mon OTC medications (Figure 6). Despite the presence of excipients that lead to prominent yet unidentified peaks, a standard Primesep 200 reversed-phase column was used to separate the major constituents of these drugs.
Miniature analyzer SIELC also offers an Alltesta Mini for applications that are even more size-
sensitive. In contrast to the full-sized analyzer, which measures 8” × 13” × 7” and weighs 22 lb, the 6-lb Mini is 8” × 8.5” × 9”. Offering the same technol- ogy and method-development library as the larger version, Alltesta Mini is suited for the following applications:
• In-home water supply testing • Onsite assessment of chemically contaminated areas
b
• Field analysis involving situations in which samples must be transported repeatedly to and from a laboratory for analysis in the absence of an onsite testing device such as the analyzer, or samples that require refrigeration, insulation, etc., and complicate the transportation process due to the added bulkiness of these materials
• In-process monitoring of production in pharmaceutical and chemical manufacturing facilities
• Teaching the fundamentals of chromatography to college students using a hands-on approach to provide real-world experience in method development and HPLC analysis.
Figure 4 – a) Relative abundance of Allura Red AC in soft drinks: 1) Powerade Zero fruit punch (The Coca-Cola Company, Atlanta, Ga.), 2) Nice! black raspberry (Walgreens, Deerfield, Ill.), 3) Sparkling Ice Cherry Limeade, 4) Sparkling Ice Pink Grapefruit (Sparkling Ice, Preston, Wash.), 5) Mountain Dew Live Wire (Pepsico, Purchase, N.Y.). Column: Obelisc N, 3.2 × 50 mm; mobile phase: MeCN 50%, AmFm pH 3.0 70 mmol; flow rate: 0.6 mL/min; detection: 525 nm; sample preparation: 1 mg/mL in 1:1 MeCN:water. b) Relative abundance of Allura Red AC in over-the- counter drugs: 1) Walgreens Nighttime Cough Relief, 2) Robitussin Cough & Cold (Pfizer, New York, N.Y.), 3) Mucinex Children’s Multi-Symptom Cold (Reckitt Benckiser, Parsippany, N.J.), 4) Robitussin Cough & Chest, 5) Children’s Mucinex Multi-Symptom Cold Nighttime. Column: Obelisc N, 3.2 × 50 mm; mobile phase: MeCN 50%, AmFmpH 3.0 70 mmol; flow rate: 0.6 mL/min; injection: 100 μL; detection: 525 nm; sample preparation: 1 mg/mL in 1:1 MeCN:water.
AMERICAN LABORATORY 28
Figure 5 – Separation of a complex mixture of nine antibiotics: 1) sul- fanilamide, 2) sulfaguanidine, 3) sulfadiazine, 4) norfloxacin, 5) tylosin, 6) josamycin, 7) dapsone, 8) sulfaquinoxaline, 9) flumequine. Column: Obelisc R, 4.6 × 150 mm; mobile phase: MeCN 0–25% in 6 min, then 70% for 14 min; formic acid 0.05–0.3% in 6 min, 14 min hold; flow rate: 1.0 mL/ min; detection: 270 nm; sample preparation: 1 mg/mL in 1:1 MeCN:water.
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