AL
Analyzing adulterants in foods Keeping foods and medications safe is a key objective, and one that is an
ongoing challenge. It can be addressed with a combination of evapora- tive light-scattering detection (ELSD) and other technologies, such as ultraviolet (UV) detection and mass spectrometry (MS). “The combina- tion of ELSD with UV detection and MS detection allows for a more complete profile of samples to understand products and any impurities that are found in the samples,” says Viet Pham, senior product marketing manager at Waters (Milford, Mass.).
One nutraceutical designed to improve the health of joints often con- tains Vitamin D3 and glucosamine. “The properties of these compounds make simultaneous analysis difficult,” Pham says. “Vitamin D3 is highly nonpolar and UV-absorbing, and glucosamine is highly polar with no chromaphore, and each component is typically present in very different concentrations.” ELSD plus MS analysis can measure Vitamin D3, glucos- amine and other components.
In analyzing oils, especially olive oil, scientists measure triacylglyc- erol (TAG). “Traditional HPLC methods for the analysis of TAGs are very long—up to two to three hours—and require a complicated extrac- tion technique before samples can be analyzed,” Pham says. “By using Waters ACQUITY UPC2
with multidetection—ELSD, photodiode array,
The Waters ELSD Model 2424 uses evaporative light-scattering detection to analyze many samples, including foods and medicines. (Image cour- tesy of Waters.)
MS—analysts are able to perform identification and quantitation of their samples in a single run.”
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AMERICAN LABORATORY 21 JANUARY/FEBRUARY 2017
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