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T en you can monitor second condenser (C2) current. Go to Single mode, select C2 and look what happens when you turn the Intensity button back and forth. If there is no change in current level for C2 then the trouble might be in the current regulating board. Oldrich Benada benada@biomed.cas.cz Fri Aug 28


Definitely sounds like one or more of the lenses is cutting out (check them all on Parameters page, as Oldrich suggests). We are having the same issue with our CM10 caused by insufficient water flow through the heatsinks (MRU and LMH) in the lower right of the microscope (if you feel the two hoses at almost ground level to the left of the rotary pump, one of them will be the return with very hot water). The water flows around the column (top and bottom circuits of the column in parallel), then through the MRU and LMH heatsinks, then out of the microscope (the diff. pump water circuit is in parallel with this). If the microscope is on for an extended period, as well as the MRU/LMH water causing the lenses to cut out, we also have the lower part of the column heating up (feel by hand on the surface). We are going to solve it by looping out the MRU+LMH part of the circuit, and instead feeding it a separate water supply from our chiller circuit. There is a blow-off option that can help clear this circuit. The connector for this is on the right when you have the back of the microscope off. When you pull out the connector, a switch will turn off the microscope automatically. This quick-coupler is then connected to the inlet to push out water + debris from the lenses, MRU, LMH part of the microscope’s cooling circuit. If you do this, make sure you know where the air will go when it is forced out of the return line of the microscope- we have had engineers make a fountain out the top of our chiller. We have a drain we can open. Ben Micklem ben.micklem@pharm. ox.ac.uk Fri Aug 28


If the beam is a pinpoint of light, the most likely cause is water


fl ow. You will see on the Lens Current page that all the main lens values will be close to 0. You should have water fl ow gauges that are located near the mechanical pump. T ese have magnetic fl oats in them and if the fl ow is low, they will trip the sensor located near the bottom of the gauge which will shut all the lenses off . T ese gauges only read the fl ow going to the upper and lower column. I would install two more gauges that read the water fl ow to the ODP and the Electronics. T e proper water fl ow through each leg is 0.7 liters/ minute. John Schreiber js51@ princeton.edu Fri Aug 28


Also wanted to mention that there are regulators that you can you use to adjust the water fl ow to the diff erent legs of the water circuit. If you have those max out, then you will need to determine what is blocking each circuit. John Schreiber js51@princeton.edu Fri Aug 28


C1


Obj Diff


Interm Proj1 Proj2


424/294 110/115 787/549 3/3


2190/1522 1789/1248


424/424 mA 120/117 787/795 3/124 5/4


−1/−1


My question is: is there any way to check the cooling hose from outside of the column which links to the projector lens part? Except for the bad cooling water for the lenses, it also could be the problem is related to the failure of a C2 condenser board, projector lens fuse, or dirty beam


 


I don’t know if the fl oat meters were a later modifi cation on CM10s, but ours doesn’t have them. It just uses a temperature sensor in the heatsinks in the electronics part of the circuit. Our CM100 has all the fl oat meters as you describe. Ben Micklem ben.micklem@ pharm.ox.ac.uk Fri Aug 28 Yes, you are correct. T e early CM10’s did not have a site glass for


the water fl ow. In which the water had only two splits in the circuit. From the water input, half went through the ODP and half goes thru the Lens and electronics. T e Power Booster safety circuit shuts off the lens if the heatsink get too hot. I would still recommend adding the fl ow gauges to adjust the proper water fl ow. John Schreiber js51@ princeton.edu Fri Aug 28


First of all I want to thank you for the prompt replies regarding the pinpoint beam issue on our CM10. In summary, the majority of comments considered that the bad water fl ow led to lens current interrup- tions intermittently due to the lens power heat sink’s overheating. T is is most likely what is happening in our CM10. Our CM10 has been with cooling issues for years. We have replaced a circulating water chiller for the system, and also monitor the temperature for the column with temperature-sensor-type stickers. We have used CLR to clean the cooling system regularly (last time was half year ago)—despite this, the temperature of the bottom part of the gun column is still high, around 82–96°F (28–35°C) during operation (e.g. 80 kV). T e temp setting for the chiller is at 14°C now. Defi nitely there is still some clog in the cooling system. T e top part of the column temperature used to be OK (always around 20°C) but today it is 28°C. Anyhow I’ll fi rst do the CLR fl ush (at least overnight) to try to boost water fl ow. When I checked the lens current, it indicated that Projector (1&2) lens were down. Here are the readings (twice) when beam was normal and pinpoint:


 


 


 


 2015 November • www.microscopy-today.com


 


  


 


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