BioScience AFM
Figure 3 : Proper integration of AFM and inverted optical microscope allows accurate co-localization of all datasets. Microtubules in HeLa cells (a) labeled with Alexa-647 antibodies in buffer solution from direct stochastic optical reconstruction microscopy (dSTORM). (b) Overlay of AFM surface data and the dSTORM image allows the correlation of cell membrane surface texture (from AFM) and the positions of the microtubules (from dSTORM). The z -scale of the AFM image is 400 nm.
Results
Submolecular structure of soft biological molecules . Specimens for AFM work, and particularly biosamples, are often analyzed at near-room or slightly elevated tempera- tures. All AFM systems are prone to drift caused by different thermal expansion of the construction materials used and thus are susceptible to external environmental heat dissipation [ 11 ]. Unlike conventional configurations that require long scanning times of at least a few minutes, the application of fast-scanning AFM mitigates this problem, which is of particular importance in the case of very sensitive and quickly deteriorating samples.
2015 November •
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Figure 4 : Fast-scanning AFM. (a) AFM image of lambda phage DNA recorded at 15 Hz line rate showing the major (labeled with red) and minor grooves (shown here in blue) in the double-helical repeat of 3.4 nm. (b) The subtrimeric structure of bacteriorhodopsin can be visualized using a line rate of 30 Hz in 10 mM TRIS (150 mM KCl) buffer. (c) A 3D view of a “1BRR” PDB trimer/lipid complex from
www.rcsb.org given for comparation. The z-scales in (a) and (b) are 3 nm and 0.4 nm, respectively.
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