Volume 21 Number 5 October 2015


table of contents preview INTRODUCTION TO EMAS SPECIAL ISSUE


Electron Probe Micro-Analysis of Materials Today. Rare and Noble elements: From Ore Deposits to High-Tech Materials


Romano Rinaldi and Federica Zaccarini EMAS SPECIAL ISSUE


Electron Probe Microanalysis: A Review of the Past, Present, and Future Romano Rinaldi and Xavier Llovet


Electron Microprobe and Raman Spectroscopy Investigation of an Oxygen-Bearing Pt–Fe–Pd–Ni–Cu Compound from Nurali Chromitite (Southern Urals, Russia) Federica Zaccarini, Giorgio Garuti, Ronald J. Bakker, and Evgeny Pushkarev


Effi cient and Accurate Identifi cation of Platinum-Group Minerals by a Combination of Mineral Liberation and Electron Probe Microanalysis with a New Approach to the Offl ine Overlap Correction of Platinum- Group Element Concentrations


Inga Osbahr, Joachim Krause, Kai Bachmann, and Jens Gutzmer


Electron Probe Microanalysis of REE in Eudialyte Group Minerals: Challenges and Solutions Petya Atanasova, Joachim Krause, Robert Möckel, Inga Osbahr, and Jens Gutzmer Age Dating from Electron Microprobe Analyses of U, T , and Pb: Geological Advantages and Analytical Diffi culties


John F.W. Bowles


Diagenetic Evolution and Reservoir Quality of Sandstones in the North Alpine Foreland Basin: A Microscale Approach


Doris Gross, Marie-Louise Grundtner, David Misch, Martin Riedl, Reinhard F. Sachsenhofer, and Lorenz Scheucher


Quantitative Microanalysis of (1–x)Pb(Mg1/3Nb2/3)O3 × xPbTiO3 (PMNT) Ferroelectric Ceramics Zoran Samardžija


Nonlinear Lock-In Infrared Microscopy: A Complementary Investigation Technique for the Analysis of Functional Electroceramic Components


Michael Hofstätter, Nadine Raidl, Bernhard Sartory, and Peter Supancic


Copper Refi nement from Anode to Cathode and then to Wire Rod: Eff ects of Impurities on Recrystallization Kinetics and Wire Ductility


Anne-Laure Helbert, Alice Moya, Tomas Jil, Michel Andrieux, Michel Ignat, François Brisset, and T ierry Baudin


Metals in Human Gall, Bladder, and Kidney Stones Based on an Electron Microprobe Investigation Reinhard Moser, Federica Zaccarini, Waltraud Moser, Rudolf Schrittwieser, and Reinhold Kerbl


EQUIPMENT AND SOFTWARE DEVELOPMENT


Diluvian Clustering: A Fast, Eff ective Algorithm for Clustering Compositional and Other Data Nicholas W. M. Ritchie


An Effi cient and Cost-Eff ective Method for Preparing Transmission Electron Microscopy Samples from Powders


Haiming Wen, Yaojun Lin, David N. Seidman, Julie M. Schoenung, Isabella J. van Rooyen, and Enrique J. Lavernia Analyzing the Eff ect of Capillary Force on Vibrational Performance of the Cantilever of an Atomic Force Microscope in Tapping Mode with Double Piezoelectric Layers in an Air Environment Amir Nahavandi and Moharam H. Korayem Gravitational-Like Lens Based on Graphene Ripple Daqing Liu, Shuyue Chen, Ning Ma, Xiang Zhao, and Zhuo Xu


A Compact “Water Window” Microscope with 60 nm Spatial Resolution for Applications in Biology and Nanotechnology


Przemyslaw Wachulak, Alfi o Torrisi, Muhammad F. Nawaz, Andrzej Bartnik, Daniel Adjei, Šárka Vondrová, Jana Turňová, Alexandr Jančarek, Jiří Limpouch, Miroslava Vrbová, and Henryk Fiedorowicz


BIOLOGICAL APPLICATIONS


Robot-Guided Atomic Force Microscopy for Mechano-Visual Phenotyping of Cancer Specimens Wenjin Chen, Zachary Brandes, Rajarshi Roy, Marina Chekmareva, Hardik J. Pandya, Jaydev P. Desai, and David J. Foran Ultrastructural Analysis of in Vivo Hypoglycemiant Eff ect of Two Polyoxometalates in Rats with Streptozotocin-Induced Diabetes


Ştefana Bâlici, Modeste Wankeu-Nya, Dan Rusu, Gheorghe Zsolt Nicula, Mariana Rusu, Adrian Florea, Horea Matei


Comparative Assessment of Oral Mesenchymal Stem Cells Isolated from Healthy and Diseased Tissues Emöke Páll, Adrian Florea, Olga Soriţău, Mihai Cenariu, Adrian S. Petruţiu, and Alexandra Roman Scanning Electron Microscopy Evaluation of Dental Root Resorption Associated With Granuloma Manila Chieruzzi, Stefano Pagano, Carlo De Carolis, Stefano Eramo, and José M. Kenny Nanoleakage in the HL and Acid-base Resistant Zone at the Adhesive Dentin Interface Toru Nikaido, Hamid Nurrohman, Tomohiro Takagaki, Alireza Sadr, Shizuko Ichinose and Junji Tagami Organic Residues on Unavailable Specimens: An Evaluation of the Use of Synthetic Replicas for SEM Identifi cation of Bloodstains (with Emphasis on Archeological and Ethnographic Artifacts) Policarp Hortolà


Absorption and Phase Contrast X-Ray Imaging in Paleontology Using Laboratory and Synchrotron Sources Pidassa Bidola, Marco Stockmar, Klaus Achterhold, Franz Pfeiff er, Mírian L.A.F. Pacheco, Carmen Soriano, Felix Beckmann, and Julia Herzen Contribution of Light and Electron Microscopy to Identifi cation of Bark from Frangula azorica, an Azorean Medicinal Plant


Maryam Malmir, Cátia Curica, Elsa T. Gomes, Rita Serrano, and Olga Silva Structural Insight into Cell Wall Architecture of Micanthus sinensis cv. using Correlative Microscopy Approaches


Jianfeng Ma, Xunli Lv, Shumin Yang, Genlin Tian, and Xing’e Liu


Microscopy Characterization of Silica-Rich Agrowastes to be used in Cement Binders: Bamboo and Sugarcane Leaves


Josefa Roselló, Lourdes Soriano, M. Pilar Santamarina, Jorge L. Akasaki, José Luiz P. Melges, and Jordi Payá


MATERIALS APPLICATIONS


Quantitative Electron-Excited X-Ray Microanalysis of Borides, Carbides, Nitrides, Oxides, and Fluorides with Scanning Electron Microscopy/Silicon Driſt Detector Energy-Dispersive Spectrometry (SEM/SDD-EDS) and NIST DTSA-II Dale E. Newbury and Nicholas W. M. Ritchie


Numerous Iron-Rich Particles Lie on the Surface of Erionite Fibers from Rome (Oregon, USA) and Karlik (Cappadocia, Turkey)


Alessandro Croce, Mario Allegrina, Caterina Rinaudo, Giovanni Gaudino, Haining Yang, and Michele Carbone


Interferometric Diff raction from Amorphous Double Films Aram Rezikyan, James A. Belcourt, and Michael M. J. Treacy


μCT-Based Analysis of the Solid Phase in Foams: Cell Wall Corrugation and other Microscopic Features Samuel Pardo-Alonso, Eusebio Solórzano, Jerome Vicente, Loes Brabant, Manuel L. Dierick, Ingo Manke, Andr Hilger, Ester Laguna, and Miguel Angel Rodriguez-Perez


Microscopy and Microanalysis website: http:journals.cambridge.org/MAM Indexed in Chemical Abstracts, Current Contents, BIOSIS, and MEDLINE (PubMed)


2015 September • www.microscopy-today.com doi: 10.1017/S1551929515000711 65 Dear Abbe,


I have an odd problem. I am studying predator-prey interactions between free-living amoebae and their prey microzoans, but it appears that how I look at the culture dishes affects my results (this seems disturbingly quantum). If I use differential-interference contrast, the amoebae successfully “stalk” and capture their prey, but if I use amoebae that express GFP, then the prey micro-critters seem to detect them and flee. Nothing else is different. What could be going on? Baffl ed in Bismarck


Dear Baffl ed,


I sympathize with you. I also have many odd problems. Most can be solved with a good Schnapps or just avoiding the relatives, though. Your problem is different, but really, the answer is right under your nosepiece. I am sorry to say you do not have a Nobel Prize coming for your discovery of a new, biological quantum mechanical effect. Instead, you have obviously forgotten that many micro-critters have photoreceptors—eyespots, sometimes even almost eyes. When you are watching your GFP-labeled amoebae, the prey critters can clearly see the glowing amoebae approaching and run away. I know I do when I see large, glowing masses approaching me. Usually after the Schnapps. The answer really is simple: quit using GFP and use CamFP, camouflage fluorescent protein. Keep in mind that there is ambient light in the natural environment of both your amoeboid predators and their prey. By using GFP, you make the amoebae stand out to any prey with micro-eyes. If you instead use CamFP, the amoebae will blend in with their surroundings and once again be able to “stalk” their prey, although, ja, there will be certain difficulties imaging them. I’m surprised you haven’t heard of CamFP. Many people doing confocal and multi- photon microscopy are using it. At least, I hope they are, given the claims made about the images they present.


If you are stumped and need a new paradigm, contact Herr Abbe for random thoughts about your problem. Contact is made through his faithful assistant at jpshield@uga.edu.


Dear Abbe


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68