bringing correlative light and electron microscopy forward
CryoCapsule ®
Designed to accelerate, facilitate and standardize sample manipulations throughout the CLEM workflow.
EMS has it! CryoCapsule
®
Sample Preparation Device for Correlative Light to Electron Microscopy
The CryoCapsule® is
a new tool in the field of High Pressure Freezing (HPF) and correlative light and electron microscopy (CLEM). Comparable to a small petri dish, it is composed of a landmarked sapphire disc and a gold spacer ring (50µm thick) maintained together by a plastic ring[1]
.
The specimens are encapsulated between the support sapphire disc (carbon landmarked) and a covering sapphire disc.
The CryoCapsule® is loaded into a specific
adaptor and live cell imaging can then be done directly on the specimen prior to HPF.
Post-HPF, the specimen is processed for freeze substitution[2] temperature sectioning.
and room
CryoCapCell has also developed a set of tools to manipulate the CryoCapsule® [2] most scientific environments.
in
CONTACT US FOR MORE INFORMATION...
References 1.
2.
Heiligenstein X, Heiligenstein J, Delevoye C, Hurbain I, Bardin S, Paul-Gilloteaux
P, Sengmanivong L, Régnier G, Salamero J, Antony C, Raposo G. The CryoCapsule: Simplifying Correlative Light to Electron Microscopy. Traffic [Internet] 2014 [cited 2014 May 14];15:700-16.
Heiligenstein X, Hurbain I, Delevoye C, Salamero J, Antony C, Raposo G. Step by step manipulation of the CryoCapsule with HPM high pressure freezers. Methods Cell Biol [Internet] 2014 [cited 2014 Nov 27];124:259-74.
P.O. Box 550 • 1560 Industry Rd. Hatfield, Pa 19440 Tel: (215) 412-8400 Fax: (215) 412-8450 email:
sgkcck@aol.com or
stacie@ems-secure.com
www.emsdiasum.com
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