Volume 21


Number 4 August 2015


table of contents preview


BIOLOGICAL APPLICATIONS AND TECHNIQUES Ultrastructural Visualization of Vaccine Adjuvant Uptake in vitro and in vivo Fabiola Giusti, Anja Seubert, Rocco Cantisani, Marco Tortoli, Ugo D’Oro, Ilaria Ferlenghi Romano Dallai and Diego Piccioli


Simultaneous Observation of Cells and Nuclear Tracks from T e Boron Neutron Capture Reaction by UV-C Sensitization of Polycarbonate


Agustina Portu, Andrés Eugenio Rossini, Silvia Inés T orp, Paula Curotto, Emiliano César Cayetano Pozzi, Pablo Granell, Federico Golmar, Rómulo Luis Cabrini, and Gisela Saint Martin Bone Diagenesis and its Implication for Disease Diagnosis: T e Relevance of Bone Microstructure Analysis for T e Study of Past Human Remains Sandra Assis


Characterization of the Distance Relationship between Localized Serotonin Receptors and Glia Cells on Fluorescence Microscopy Images of Brain Tissue


Jaroslaw Jacak, Susanne Schaller, Daniela Borgmann, and Stephan Winkler Tracing CD34+ Stromal Fibroblasts in Palatal Mucosa and Periodontal Granulation Tissue as a Possible Cell Reservoir For Periodontal Regeneration


Alexandra Roman, Emöke Páll, Carmen M. Mihu, Adrian S. Petruţiu, Lucian Barbu-Tudoran, Radu S. Câmpian, Adrian Florea, and Carmen Georgiu Bond Strength of High-Viscosity Glass Ionomer Cements is Aff ected By Tubular Density and Location in Dentin?


Tamara Kerber Tedesco, Ana Flávia Bissoto Calvo, Gabrielle Gonsalli Domingues, Fausto Medeiros Mendes, and Daniela Prócida Raggio


Phase and Texture Characterizations of Scar Collagen Second Harmonic Generation Images Varied with Scar Duration


Guannan Chen, Yao Liu, Xiaoqin Zhu, Zufang Huang, Jianyong Cai, Rong Chen, Shuyuan Xiong, and Haishan Zeng


Automatic Evaluation of Collagen Fiber Directions From Polarized Light Microscopy Images Kamil Novak, Stanislav Polzer, Michal Tichy, and Jiri Bursa


A Refi ned Single Particle Reconstruction Procedure to Process Two Dimensional Crystal Images from Transmission Electron Microscopy Qie Kuang, Pasi Purhonen, T irupathi Pattipaka, Yohannes Haileyesus Ayele, Hans Hebert and Philip J.B. Koeck


Reliable Detection and Smart Deletion of Malassez Counting Chamber Grid in Microscopic White Light Images for Microbiological Applications


Emmanuel Denimal, Ambroise Marin, Stéphane Guyot, Ludovic Journaux, and Paul Molin Cryostat Slice Irregularities May Introduce Bias In Tissue T ickness Estimation – Relevance For Cell Counting Methods


A. Puigdellívol-Sánchez, A. Giralt, A, Casanovas, J. Alberch, and A. Prats-Galino A Method For Preparing Diffi cult Plant Tissues For Light And Electron Microscopy Peta L. Clode


MATERIALS APPLICATIONS AND TECHNIQUES


Voltage and Dopant Concentration Measurements of Semiconductors Using a Band-Pass Toroidal Energy Analyzer Inside a Scanning Electron Microscope Avinash Srinivasan and Anjam Khursheed Electron Backscatter Diff raction and Transmission Kikuchi Diff raction Analysis of an Austenitic Stainless Steel Subjected to Surface Mechanical Attrition Treatment and Plasma Nitriding Gwénaëlle Proust, Delphine Retraint, Mahdi Chemkhi, Arjen Roos, and Clemence Demangel Characterization of Sputtered CdTe T in Films with Electron Backscatter Diff raction and Correlation with Device Performance


M.M. Nowell, M.A. Scarpulla, N.R. Paudel, K.A. Wieland, A.D. Compaan, and X. Liu


T e Internal Structure of Macroporous Membranes and Transport of Surface-Modifi ed Nanoparticles Sang Joon Lee, Kiwoong Kim, and Sungsook Ahn


Accurate Nanoscale Crystallography in Real-Space Using Scanning Transmission Electron Microscopy J.H. Dycus, J.S. Harris, X. Sang, C.M. Francher, S.D. Findlay, A.A. Oni, T.E. Chan, C.C. Koch, J.L. Jones, L.J. Allen, D.L. Invring, and J.M Lebeau


Solid State Reaction Mechanism and Microstructure Evolution of Ni-Al Powders during High Energy Ball Milling Revisited by TEM


G.H. Fan, L.Geng, Y.C. Feng, X.P. Cui, and X.D.Yan


Characterization of Darai Limestone Composition and Porosity Using Data-Constrained Modelling and Comparison with Xenon K-Edge Subtraction Imaging


Sheridan C Mayo, Y. Sam Yang, Marina Pervukhina, Michael Ben Clennell, Lionel Esteban, Sally Irvine, Karen Siu, Anton Maksimenko, and Andrew Tulloh Evaluating Deformation-Induced Grain Orientation Change in a Polycrystal During In-Situ Tensile Deformation using EBSD


EBSDinterp 1.0: A MATLAB® Program to Perform Microstructurally Constrained Interpolation of EBSD data


T.E. Buchheit, J.D. Carroll, B.G. Clark, and B.L. Boyce Mark A. Pearce


Quantitative Chemical Mapping of InGaN Quantum Wells (QW) from Calibrated High Angle Annular Dark Field (HAADF) Micrographs


Daniel Carvalho, Francisco M. Morales, Teresa Ben, Rafael García, Andrés Redondo-Cubero, Eduardo Alves, Katharina Lorenz, Paul R. Edwards, Kevin P. O’Donnell, and Christian Wetzel iSpectra: An Open Source Toolbox for T e Analysis of Spectral Images Recorded on Scanning Electron Microscope Christian Liebske


Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles Sean K. Mulligan, Jeff rey A. Speir, Ivan Razinkov


Electron Correlation Microscopy: A New Technique for Studying Local Atom Dynamics Applied to a Supercooled Liquid


L. He, P. Zhang, M.F. Besser, M. J. Kramer, and P. Voyles T eory and New Applications of ex situ Liſt Out


Lucille A. Giannuzzi, Zhiyang Yu, Denise Yin, Martin P. Harmer, Qiang Xu, Noel S. Smith, Lisa Chan, Jon Hiller, Dustin Hess, and Trevor Clark


BOOK REVIEWS


Practical Materials Characterization. Mauro Sardela (Ed.) by Fumiya Watanabe


Optical Nanoscopy and Novel Microscopy Techniques. Peng Xi (Ed.) by Guy Cox


Microscopy and Microanalysis website: http:journals.cambridge.org/MAM Indexed in Chemical Abstracts, Current Contents, BIOSIS, and MEDLINE (PubMed)


2015 July • www.microscopy-today.com


Dear Abbe, I have trouble with some of my TEM sections. T e sections keep disintegrating or “bursting” under the beam, and I cannot obtain an image. Any solutions? Annoyed in Antigua


Dear Annoyed,


Quit your whining! At least it’s only some of the sections. I remember starting out in EM when our samples were so thick that we could only look in the holes and pretend there was information! I began working on sample preparation with Ernst Ruska and Bodo von Borries while snacking over Käse-igel and lagers. It was problematic until I began perusing papers by Keith Porter—who was working on perfecting an ultramicrotome and associated techniques. Since we were viewing holes anyway, I approached Dr. Porter with the idea of creating a Schrödinger ultramicrotome. T is microtome would produce a quantum superposition of sections—simultaneously having sample and not having sample in the sections. You may be having a similar situation: a Verschränkung of simultaneous bursting and non-bursting states of the sections. You’ll never know until you look in the scope, but then it’ll be too late, or not late enough…


Dear Abbe,


I’m having some issues cutting ultrathin sections embedded in Felix Katt’s resin formulation. I started thinking about what happens at the atomic level when the diamond knife cuts a section, how it breaks the bonds of the material being cut, and how the electrons of atoms at the knife edge interact with the specimen atoms’ electrons, and I got a headache. What really happens to the sections produced? How frayed are the section faces? Tattered in Tucson


Dear Tattered,


It’s all quantum. Don’t worry about what happens since you can’t really know. I am still in the process of designing the new Schrödinger brand ultramicrotome (see my response to “Agitated” above), which uses a laser beam instead of a diamond knife to cut the sections. T is works nicely since it’s a much simpler mechanism. T e laser beam moves, and the specimen block is stationary. Unfortunately, in our trial runs a quantum particle, the photon, disrupts the interactions of other quantum particles (the electrons in the atomic bonds), so there’s no way to know if anything has happened until the section has been cut or not cut. You’re making things more diffi cult by using Katt’s resin. T is is well known, amongst those who use it, for either cutting or not cutting, but you can’t know which until you look for the section. I usually fi nd it best to wait for the knife boat water waves to collapse and to not look for the sections until aſt er I’ve had a suffi ciency of schnapps so that I’m seeing double. T en the sections can be seen in both the “there” and “not-there” states at the same time. T e trick is picking up the “there” sections.


Having metaphysical issues with samples? Ontological issues with coworkers? Let Herr Abbe have a philosophical swipe at them! jpshield@uga.edu.


doi: 10.1017/S1551929515000504 81


Dear Abbe


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