Carmichael’s Concise Review
Coming Events 2015
Stereology & Image Analysis July 6–10, 2015 Liege, Belgium
www.14icsia.com
Semicon West, 2015 July 14–16, 2015 San Francisco, CA
www.semiconwest2015.org
Microscopy & Microanalysis 2015 August 2–6, 2015 Portland, OR
www.microscopy.org
31st European Conference on Surface Science (ECOSS-31) August 31–September 4, 2015 Barcelona, Spain
www.ecoss2015.org
SMMS 2015: Single-Molecule Microscopy
and Spectroscopy September 14–16, 2015 London, UK
www.rsc.org/conferencesandevents/rscconferences/fd/ molecule-fd2015/
index.asp
SPIE Scanning Microscopies September 29–October 1, 2015 Monterey, CA
http://spie.org/x104030.xml
Materials Science & Technology 2015 October 4–8, 2015 Columbus OH
http://matscitech.org
Neuroscience 2015 October 17–21, 2015 Chicago, IL
Sponsor: Society for Neuroscience
www.sfn.org
2015 MRS Fall Meeting & Exhibition November 29–December 4, 2015 Boston, MA
Sponsor: Materials Research Society (MRS)
www.mrs.org/fall2015
American Society for Cell Biology (ASCB)
2015 Annual Meeting December 12–16, 2015 San Diego, CA
http://ascb.org/future-ascb-annual-meetings 2016
Microscopy & Microanalysis 2016 July 24–28, 2016 Columbus, OH
www.microscopy.org
2017
Microscopy & Microanalysis 2017 July 23–27, 2017 St. Louis, MO
www.microscopy.org 2018
Microscopy & Microanalysis 2018 August 5–9, 2018 Baltimore, MD
www.microscopy.org
2019
Microscopy & Microanalysis 2019 August 4–8, 2019 Portland, OR
www.microscopy.org
More Meetings and Courses Check the complete calendar near the back of this magazine.
8
Visualizing a Molecular Motor Stephen W. Carmichael Mayo Clinic , Rochester , MN 55905
carmichael.stephen@
mayo.edu
Dynactin works with the cytoplasmic dynein-1 motor (dynein) to transport cargo along the microtubule-based skeleton of cells. Together, these protein complexes maintain the spatial organization of the cell, return components from the periphery of the cell, and assist with cellular division. Although much is known about dynactin and dynein, a number of questions remain. For example, how does dynein bind to dynactin, and why does the interaction require the cargo adaptor Bicaudal-D2 (BICD2)? To address this and related questions, a team in the U.K. lead by Linas Urnavicius, Kai Zhang, Aristides Diamant, and Andrew Carter [ 1 ] took advantage of recent advances in cryo-electron microscopy (cryo-EM) to improve the understanding of the structure of dynactin.
For a number of technical reasons, dynactin is a challenging target for cryo-EM. Urnavicius et al. overcame the hurdles by making cryo-EM maps at resolutions between 6.5 Å and 3.5 Å and used these maps to build a model of dynactin. The dynactin filament is nine subunits long and consists of two protofilaments that wrap around each other. The presence of β -actin in the filament was controversial. The cryo-EM map that they constructed was of sufficient quality to show that one
Figure 1 : Model of the structure of cytoplasmic dynein (gray) bound to dynactin (multicolor) via the Golgi vesicle cargo adaptor BICD2 (orange) constructed from cryo-EM maps.
doi: 10.1017/S1551929515000619 2015 July
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