Volume 23 23


Number 4 Number


20


2015 July July


Light Microscopy Contents


12 Digital Staining: Microscopy of Live Cells Without Invasive Chemicals Lisa Pollaro, Bastien Dalla Piazza, and Yann Cotte


18 A Rapid Image Acquisition Method for Focus Stacking in Microscopy Douglas Clark and Brian Brown


26 Three-Dimensional Printing of Super-Resolution Microscopy Images Emily M. Mace, Jesse Moon, and Jordan S. Orange


30 Using Refl ectance Transformation Imaging and 3D Laser Scanning Confocal Microscopy to Evaluate Relief and Contour Lines on Ancient Attic Greek Vases Paula Artal-Isbrand and Philip Klausmeyer


36 A Flexible and Economical Field Microscope Glenn Shipley and Robert Hoelter


Microanalysis About the Cover


44 Atomic Elemental and Chemical Analysis of SrTiO3/LaMnO3 Multilayers Using Fast Simultaneous EELS and EDS Analysis in DigitalMicrograph Paolo Longo, Paul J Thomas, Aziz Aitouchen, Phil Rice, Teya Topuria, and Ray D Twesten


Microscopy 101


54 Strategies to Effi ciently Locate Cultured Cells of Interest on Transmission Electron Microscopy Grids Linda White, Jeffrey Prince, and Kathryn Tosney


Opinion


58 A Challenge to Microscopists Alwyn Eades


Clockwise from upper left: digitally stained mouse fi broblastic reticular cell in the process of dying. Elapsed time = 9.8 minutes. Full width = 57 µm.


See article by Pollaro et al. Departments


7 Editorial 8 Carmichael’s Concise Review


60 Pioneers 62 Industry News 64 Product News


66 NetNotes 76 Calendar of Meetings 81 Dear Abbe 82 Index of Advertisers


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