GLYCOXIDATIVE MODIFICATION OF ADENOSINE continued


Figure 2 – LC/MS spectrum using multiple reaction monitoring (MRM) spectrometric analysis of a) CMAd, b) CMAd in fasting human urine sam- ple and c) CMAd and urine mixing experiment.


Figure 3 – LC/MS spectrum using MRM spectrometric analysis of a) CMAd, b) CMAd in fasting human urine sample and c) CMAd and urine mixing experiment.


and diabetic. Descriptive statistical analyses were performed on each clinical parameter to obtain descriptive statistics such as mean, median and standard deviation (SD), as well as minimum and maximum values for the three groups. The General Linear Model test applied analysis of vari- ance (ANOVA) followed by the adjusted Tukey’s test as a post hoc test. The F-test was used to prove the null hypothesis that there was no significant difference among the three groups, while the Tukey’s test compared each clinical parameter between the two groups. Results were tested at .05, .01 and .001 levels of significance.


Figure 1 – LC-MS/MS-ESI (TIC [total ion chromatograms] and ion plots) spectrum of adenosine with a) D-glucose, b) D-ribose and c) chloroacetic acid.


Results Table 1 illustrates the results of the ANOVA and Tukey’s tests on BMI, fasting


BGL and HbA1c using mean ±SD. The symbols *, ** and †† indicate the differences among the groups and their levels of significance.


AMERICAN LABORATORY • 36 • AUGUST 2015


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