AUTOMATED SOLID-PHASE EXTRACTION continued Table 3 – Comparison of manual and automated SPE
Manual SPE: 12 samples
LOD (µg/L) LOQ (µg/L)
6.85 13.12 Measuring precision (%) 91.58 ± 2.61
Repeatability precision (%) 95.26 ± 4.14 97.63 ± 4.3 Recovery rate (%)
Within-day precision (%) Day 1 Day 2 Day 3 Day 4 Day 5
90.10–104.26 88.60–108.10
93.64 ± 3.33 97.63 ± 4.39 99.10 ± 4.85 98.98 ± 5.32 95.33 ± 3.48 99.15 ± 6.23 99.24 ± 5.28 94.41 ± 6.46 92.88 ± 4.58 100.04 ± 5.22
Automated SPE:
24-column adapter plate 6.26
6.77 96.09 ± 1.44
74.35 ± 5.89 72.88–95.20
74.35 ± 5.89 74.78 ± 6.35 83.40 ± 6.07 80.83 ± 5.68 77.44 ± 6.32
Automated SPE: 96-well plate
Conclusion The positive pressure unit described in this article can replace the manual process as well as vacuum systems while achieving reliable results. Advantages include higher applicable pressure on the columns than vacuum systems, uniform pressure over all the columns and complete and consistent sample processing. Consequently, the automated SPE method with positive pressure has a defi ned working time due to complete sample processing. In contrast with the vacuum method, the pro- cessing time needs to be extended to ensure fully processed samples. During this extended processing time, already emptied columns dry out, which negatively impacts the SPE and the results generated.
In conclusion, the positive pressure unit deliv- ers optimized automated SPE with high sample throughput, which can be applicable on the industrial scale.
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AMERICAN LABORATORY • 32 • MARCH 2015
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